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Chinese Journal of Integrative Medicine

, Volume 14, Issue 3, pp 207–211 | Cite as

Effect of Radix Isatidis polysaccharides on immunological function and expression of immune related cytokines in mice

  • Yan-ling Zhao (赵艳玲)
  • Jia-bo Wang (王伽伯)
  • Li-mei Shan (山丽梅)
  • Cheng Jin (金 城)
  • Li Ma (马 莉)
  • Xiao-he Xiao (肖小河)
Experimental Research

Abstract

Objective

To investigate the effect of Radix Isatidis polysaccharides (RIP) on the immunological function and expression of immune related cytokines in mice.

Methods

Sixty mice were randomly divided into six groups, the normal group, cyclophosphamide (Cy) model group, Levamisole (positive control) group, RIP low, medium and high dose groups (0.08 g/kg, 0.16 g/kg, 0.32 g/kg, respectively), ten in each group. By detecting the value of abdominal macrophage phagocytic index, serum hemolysin (HC50), proliferation of lymphocytes and expression of related cytokines, interleukin (IL-2) and interferon γ (INF-γ), the effect of RIP on immunological function and its mechanism were studied.

Results

RIP could improve the level of hemolysin in immunological function depressed mice. The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11±0.13 to 1.42±0.26. The level of IL-2 and INF-γ could be decreased by Cy to 38.12±6.88 ng/L and 139.23±29.87 ng/L, respectively, while RIP in high dose could increase the secretion of IL-2 and INF-γ to 53.54±14.43 ng/L and 189.91±32.63 ng/L, respectively.

Conclusion

RIP could enhance non-specific immunological function, humoral immunity and cellular immunity in mice.

Key Words

Radix Isatidis polysaccharides macrophage serum hemolysin lymphocyte cytokine 

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References

  1. 1.
    Wu XY, Liu YH, Sheng WY, et al. Chemical constituents of isatis indigotica. Planta Medica 1997;63(1):55–57.PubMedCrossRefGoogle Scholar
  2. 2.
    Yin J, Guo LG. Modern research and clinical application of traditional Chinese medicine. Beijing: Academy Press, 1995:52–57.Google Scholar
  3. 3.
    Ma L, Feng SH, Tang JY, et al. Influence of Radix Isatidis polysaccharides produced by different preparation technologies on proliferation function of mouse splenic lymphocytes. China Pharm (Chin) 2007; 18(3): 169–171.Google Scholar
  4. 4.
    Wu XL, Cai YQ, Zhao Y, Li SF. Effects of extract from taraxacum monogon (ETM) on immune modulation in mice. Acta Univ Med Nanjing (Natural Sci, Chin) 2005; 25(3):163–165.Google Scholar
  5. 5.
    Liu J, Gao M, Yu H, et al. Effect of wild lactarius volemus from Guizhou province on immune function in mice. Chin J Public Health (Chin) 2007;23(3): 291–293.Google Scholar
  6. 6.
    Kim HM, Han SB, Oh GT, et al. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom phellinus Linteus. Int J Immunopharmacol 1996;18:295–303.PubMedCrossRefGoogle Scholar
  7. 7.
    Sherwood ER, Wiliams DL, McNamee RB, et al. Enhancement of interleukin 1 and interleukin 2 production by soluble glucan. Int J Immunopharmacol 1987; 9:261–267.PubMedCrossRefGoogle Scholar
  8. 8.
    Estrada A, Yun CH, Van Kessel A, et al. Immunomodulatory activities of oat beta-glucan in vitro and in vitro. Microbiol Immunol 1997;42:991–998.Google Scholar
  9. 9.
    Bin-Hafeez B, Haque R, Parvez S, et al. Immunomodulatory effects of fenugreek (Trigonella foenum graecum L.) extract in mice. Int Immunopharmacol 2003;3:257–265.PubMedCrossRefGoogle Scholar
  10. 10.
    Gong FL, ed. Medical immunology. 2nd ed. Beijing: Science Press, 2004:171–174.Google Scholar

Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Yan-ling Zhao (赵艳玲)
    • 1
  • Jia-bo Wang (王伽伯)
    • 1
  • Li-mei Shan (山丽梅)
    • 1
  • Cheng Jin (金 城)
    • 1
  • Li Ma (马 莉)
    • 1
  • Xiao-he Xiao (肖小河)
    • 1
  1. 1.Institute of Traditional Chinese Material Medicathe 302 Hospital of PLABeijingChina

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