Chinese Journal of Integrative Medicine

, Volume 14, Issue 2, pp 123–127 | Cite as

Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells

  • Shu-sheng Li (李树生)
  • Jun Feng (冯 俊)
  • Zhi Zheng (郑 智)
  • Qian-sheng Liang (梁黔生)
Experimental Research



To observe the effects of sodium tanshinone II A sulfonate (STS) on angiotensin II (Ang II)-induced hypertrophy of myocardial cells through the expression of phosphorylated extracellular signal-regulated kinase (p-ERK1/2).


In the primary culture of neonatal rat myocardial cells, the total protein content in myocardial cells was determined by coomassie brilliant blue and the protein synthesis rate was measured by [3H]-Leucine incorporation as indexes for hypertrophy of myocardial cells. The expression of p-ERK1/2 was determined using Western blot and immunofluorescence labeling.


(1) The total protein and protein synthesis rate increased significantly in contrast to the control group after the myocardial cells were stimulated by Ang II (1 μmol/L) for 24 h; STS markedly inhibited the increment of the total protein level induced by Ang II and the syntheses of protein. (2) After pretreatment of myocardial cells with Ang II (1 μmol/L) for 5 min, the p-ERK1/2 protein expression was increased, with the most obvious effect shown at about 10 min; pretreatment of myocardial cells with STS at different doses (2, 10, 50 μmol/L) for 30 min resulted in obvious inhibition of the expression of p-ERK1/2 stimulated by Ang II in a dose-dependent manner. (3) After the myocardial cells were stimulated by Ang II (1 μmol/L), the immunofl uorescence of ERK1/2 rapidly appeared in the nucleus. The activation and translocation process of ERK1/2 induced by Ang II was blocked distinctly by STS.


STS inhibited the myocardial cell hypertrophy induced by Ang II, and the mechanism may be associated with the inhibition of p-ERK1/2 expression.

Key words

sodium tanshinone II A sulfonate angiotensin II myocardial cell hypertrophy extracellular signal-regulated kinase 


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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Shu-sheng Li (李树生)
    • 1
  • Jun Feng (冯 俊)
    • 1
  • Zhi Zheng (郑 智)
    • 1
  • Qian-sheng Liang (梁黔生)
    • 1
  1. 1.Department of Emergency Internal MedicineTongji Hospital, Huazhong University of Science and TechnologyWuhanChina

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