Forestry Studies in China

, Volume 14, Issue 1, pp 1–7 | Cite as

Differences of EST-SSR and genomic-SSR markers in assessing genetic diversity in poplar

  • Yue-peng Song
  • Xi-bing Jiang
  • Man Zhang
  • Ze-liang Wang
  • Wen-hao Bo
  • Xin-min An
  • De-qiang Zhang
  • Zhi-yi Zhang
Research Article

Abstract

We analyzed the genetic differences of 16 poplar clones between genomic-SSR and EST-SSR markers. The statistical results show that the average number of alleles detected by genomic-SSR was 4.1, Shannon’s index 1.0646, observed heterozygosity 0.4427 and expected heterozygosity 0.5523, while for the EST-SSR, the average number of alleles was 2.8, Shannon’s index 0.6985, observed heterozygosity 0.2330 and expected heterozygosity 0.4684. Cluster analysis indicated that the EST-SSR capacity of genotypic identification was more precise than that of genomic-SSR. These results reveal that EST-SSR and genomic-SSR have statistically significant genetic differences in polymorphism detection and genotypic identification. These differences could provide a theoretical basis for the rational use of SSR markers in species diversity and other related research.

Key words

poplar genomic-SSR EST-SSR genetic differences 

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Copyright information

© Beijing Forestry University and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Yue-peng Song
    • 1
  • Xi-bing Jiang
    • 1
  • Man Zhang
    • 2
  • Ze-liang Wang
    • 1
  • Wen-hao Bo
    • 1
  • Xin-min An
    • 1
  • De-qiang Zhang
    • 1
  • Zhi-yi Zhang
    • 1
  1. 1.National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry AdministrationBeijing Forestry UniversityBeijingP. R. China
  2. 2.College of HorticultureNorthwest Agriculture and Forestry UniversityYanglingP. R. China

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