In Vitro Cellular & Developmental Biology - Plant

, Volume 55, Issue 6, pp 702–709 | Cite as

In vitro propagation of Trichosanthes kirilowii Maxim. through nodal segment shoot proliferation

  • Su Ji Joo
  • A. Ra Yoon
  • Yong-Goo Kim
  • Byeong Cheol Moon
  • Richard Komakech
  • Youngmin KangEmail author
Plant Tissue Culture


Trichosanthes kirilowii Maxim. is a vital traditional herbal medicinal plant found in northeastern Asia. Its roots, fruits, and seeds are used as food and medicine. Roots harvested for medicinal use take over 3 yr to mature when the plant is grown in a traditional way through cultivation in the field. This coupled with uncertainty in identification of the plant when collected from the wild calls for a standard in vitro propagation system to meet the increasing demand for it. The purpose of this study was to develop a standard protocol for the in vitro micropropagation of T. kirilowii. Ten different media supplemented with different concentrations of plant growth regulators were evaluated. At 5 wk, De Greef and Jacobs medium supplemented with 0.1 mg L−1 kinetin led to optimal shoot growth, while the same medium supplemented with 0.5 mg L−1 indole 3-butyric acid induced optimal root growth, also at 5 wk. The micropropagated plants that were acclimatized for 8 wk in the greenhouse produced mature root tubers after planted in the field for 3 mo. Therefore, these findings provide a basis for future large-scale in vitro propagation of T. kirilowii.


Biomass In vitro propagation Herbal medicine Trichosanthes kirilowii Radix Trichosanthis 



This work was supported by the Development of Foundational Techniques for the Domestic Production of Authentic Herbal Medicines based on the Establishment of Molecular Authentication System (K17403), Development of Foundational Techniques for the Domestic Production of Herbal Medicines (K18405), Development of Application Techniques for the Standardization Resources of Herbal Medicines (KSN1911420), Korea Institute of Oriental Medicine through the Ministry of Science, and ICT, Republic of Korea.


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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Herbal Medicine Resources Research CenterKorea Institute of Oriental MedicineNaju-siRepublic of Korea
  2. 2.Korea Institute of Oriental Medicine (KIOM) Campus, Korean Convergence Medicine MajorUniversity of Science & Technology (UST)DaejeonRepublic of Korea
  3. 3.Natural Chemotherapeutics Research Institute (NCRI), Ministry of HealthKampalaUganda

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