Advertisement

Iron nanoparticles and potassium silicate interaction effect on salt-stressed grape cuttings under in vitro conditions: a morphophysiological and biochemical evaluation

  • Ali Ghadakchi asl
  • Ali akbar MozafariEmail author
  • Nasser Ghaderi
Abiotic Stress Responses
  • 33 Downloads

Abstract

As two newly important components for plant tissue culture, the impacts of iron nanoparticle and potassium silicate were studied on the regeneration and growth of grape cuttings var. Khoshnaw under salinity condition. The treatments consisted of salinity stress (0, 50, and 100 mM NaCl), iron nanoparticles (0.0, 0.08, and 0.8 ppm) and potassium silicate (0, 1, and 2 mM) under an in vitro environment. The overall results indicated that salinity significantly (p ≤ 0.05) increased soluble carbohydrates and carotenoid contents. On one hand, it reduced all studied morphological and physiological traits including shoot number, shoot and root length, shoot and root fresh weight, root volume, and leaf area, along with relative water content (RWC) and chlorophylls’ content. On the other hand, the application of iron nanoparticles and potassium silicate, alone or in combination, could significantly compensate the deleterious effects of salinity on morphological traits, leading to increase their mean values compared to control condition (p ≤ 0.05). Soluble carbohydrate content showed negative significant (p ≤ 0.05) correlation with RWC, chlorophyll a, and all morphological parameters. Chlorophyll b and total chlorophyll contents showed positive significant (p ≤ 0.01) correlation with RWC. The application of higher concentrations of potassium silicate resulted in a greater ability of plants to tolerate salinity; moreover, the results suggest that moderate concentrations of iron nanoparticles may be more profitable for increasing salinity tolerance.

Keywords

Iron Carbohydrate Silicon Sodium Relative water content 

Notes

Funding information

This work was supported by the University of Kurdistan under grant number [4.14364]

References

  1. Alexandre A, Meunier JD, Colin F, Koud JM (1997) Plant impact on the biogeochemical cycle of silicon and related weathering processes. Geochim Cosmochim Acta 61:677–682CrossRefGoogle Scholar
  2. Barhoumi Z, Rabhi M, Gharsalli M (2007) Effect of two nitrogen forms on the growth and iron nutrition of pea cultivated in presence of bicarbonate. J Plant Nutr 30:1953–1965CrossRefGoogle Scholar
  3. Daub ME (1986) Tissue culture and the selection of resistance to pathogens. Annu Rev Phytopathol 24:159–186CrossRefGoogle Scholar
  4. Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42CrossRefGoogle Scholar
  5. El-Agamy SZ, El-Mahdy TK, Mohamed AA (2008) In vitro propagation of some grape rootstocks. In: Proceedings of the first international symposium on biotechnology of fruit species (Biotechfruit 2008). Dresden, Germany, September 1–5 Abstract Book, pp 125–132Google Scholar
  6. Elbotaty EMA (2012) Production of developed grape rootstocks using in vitro mutations. Dissertation, University of Cairo, EgyptGoogle Scholar
  7. Fisarakis I, Chartzoulakis K, Stavrakas D (2001) Response of Sultana vines (V. vinifera L.) on six rootstocks to NaCl salinity exposure and recovery. Agric Water Manag 51:13–27CrossRefGoogle Scholar
  8. Flowers TJ, Yeo AR (1989) Effects of salinity on plant growth and crop yield. In: Cherry JH (ed) Environmental stress in plants. Springer, Berlin, pp 101–119CrossRefGoogle Scholar
  9. George EF, Hall MA, De Klerk GJ (2007) Plant propagation by tissue culture, vol 1. Springer, BerlinCrossRefGoogle Scholar
  10. Gong H, Zhu X, Chen K, Wang S, Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci 169:313–321CrossRefGoogle Scholar
  11. Gupta R, Wall T, Baxter L (2007) Impact of mineral impurities in solid fuel combustion. Springer Science & Business Media, New YorkGoogle Scholar
  12. Habibi G, Sarvary S (2015) The roles of selenium in protecting lemon balm against salt stress. Iran J Plant Physiol 5:1425–1433Google Scholar
  13. Jaén J, de Saldana EG, Hernandez C (1999) Characterization of reaction products of iron and iron salts and aqueous plant extracts. Hyper Interact 122:139–145CrossRefGoogle Scholar
  14. Kotuby-Amacher J, Koenig R, Kitchen B (2000) Salinity and plant tolerance. Electronic Publication AG-SO-03, Utah State University Extension, Available via https://digitalcommons.usu.edu. Cited 4 Apr 2019
  15. Liang Y, Chen Q, Liu Q, Zhang W, Ding R (2003) Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J Plant Physiol 160:1157–1164CrossRefGoogle Scholar
  16. Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. In: Wrolstad RE, Acree TE, An H, Decker EA, Penner MH, Reid DS, Schwartz SJ, Shoemaker Sporns P (eds) Current protocols in food analytical chemistry (CPFA). Wiley, New York, pp F4.3.1 –F4.3.8,Google Scholar
  17. Ma JF, Takahashi E (2002) Soil, fertilizer and plant silicon research in Japan. Elsevier, AmsterdamGoogle Scholar
  18. Ma JF, Yamaji N, Mitani-Ueno N (2011) Transport of silicon from roots to panicles in plants. Proc Jpn Acad Ser B Phys Biol Sci 87:377–385CrossRefGoogle Scholar
  19. Malusá E, Sas-Paszt L, Ciesielska J (2012) Technologies for beneficial microorganisms inocula used as biofertilizers. Sci World J 2012:491206CrossRefGoogle Scholar
  20. Mozafari AA, Vafaee Y, Karami E (2015) In vitro propagation and conservation of Satureja avromanica Maroofi—an indigenous threatened medicinal plant of Iran. Physiol Mol Biol Plants 21:433–439CrossRefGoogle Scholar
  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  22. Nadi E, Aynehband A, Mojaddam M (2013) Effect of nano-iron chelate fertilizer on grain yield, protein percent and chlorophyll content of Faba bean (Vicia faba L.). Int J Biosci 3:267–272Google Scholar
  23. Nwugo CC, Huerta AJ (2008) Silicon-induced cadmium resistance in rice (Oryza sativa). J Plant Nutr Soil Sci 17:841–848CrossRefGoogle Scholar
  24. Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rose—a review. Biotechnol Adv 24:94–114CrossRefGoogle Scholar
  25. Richmond KE, Sussman M (2003) Got silicon? The non-essential beneficial plant nutrient. Curr Opin Plant Biol 6:268–272CrossRefGoogle Scholar
  26. Rodrigues F, Duarte H, Domiciano G, Souza C, Korndörfer G, Zambolim L (2009) Foliar application of potassium silicate reduces the intensity of soybean rust. Australas Plant Pathol 38:366–372CrossRefGoogle Scholar
  27. Rolli E, Brunoni F, Marieschi M, Torelli A, Ricci A (2015) In vitro micropropagation of the aquatic fern Marsilea quadrifolia L. and genetic stability assessment by RAPD markers. Plant Biosys 149:7–14CrossRefGoogle Scholar
  28. Römheld V, Marschner H (1991) Function of micronutrients in plants. In: Mortdvedt JJ, Cox FR, Shuman LM, Welch RM (eds) Micronutrients in Agriculture. Soil Science Society of America, Madisonm USA pp 297–318Google Scholar
  29. Sivanesan I, Jeong BR (2014) Silicon promotes adventitious shoot regeneration and enhances salinity tolerance of Ajuga multiflora Bunge by altering activity of antioxidant enzyme. Sci World J 2014:521703CrossRefGoogle Scholar
  30. Squashic SA, Hudy KM, Purdy DC (2012) Nutritional supplement for use under physiologically stressful conditions, Google Patents. https://patents.google.com/patent/US7901710B2/en. Accessed 2 Nov 2018
  31. Tahir MA, Rahmatullah T, Aziz M, Ashraf S, Kanwal MM, Maqsood MA (2006) Beneficial effects of silicon in wheat (Triticum aestivum L.) under salinity stress. Pak J Bot 38:1715–1722Google Scholar
  32. Taiz L, Zeiger E (2015) Plant physiology, 5th ed. Sinauer Associates, Sunderland, MAGoogle Scholar
  33. Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314:1298–1301CrossRefGoogle Scholar
  34. Ursache-Oprisan M, Focanici E, Creanga D, Caltun O (2011) Sunflower chlorophyll levels after magnetic nanoparticle supply. Afr J Biotechnol 10:7092–7098Google Scholar
  35. Vafaee Y, Ghaderi N, Khadivi A (2017) Morphological variation and marker-fruit trait associations in a collection of grape (Vitis vinifera L.). Sci Hortic 225:771–782CrossRefGoogle Scholar
  36. Van der Salm TP, Van der Toorn CJ, Hänisch ten Cate CH, Dubois LA, De Vries DP, Dons HJ (1994) Importance of the iron chelate formula for micropropagation of Rosa hybrida L‘Moneyway’. Plant Cell Tissue Organ Cult 37:73–77CrossRefGoogle Scholar
  37. Yaghubi K, Ghaderi N, Vafaee Y, Javadi T (2016) Potassium silicate alleviates deleterious effects of salinity on two strawberry cultivars grown under soilless pot culture. Sci Hortic 213:87–95CrossRefGoogle Scholar
  38. Yeo A, Yeo M, Flowers S, Flowers T (1990) Screening of rice (Oryza sativa L.) genotypes for physiological characters contributing to salinity resistance, and their relationship to overall performance. Theor Appl Genet 79:377–384CrossRefGoogle Scholar
  39. Zawadzka M, Orlikowska T (2006) The influence of FeEDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants. Plant Cell Tissue Organ Cult 85:145–149CrossRefGoogle Scholar
  40. Zhu Z, Wei G, Li J, Qian Q, Yu J (2004) Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Sci 167:527–533CrossRefGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  • Ali Ghadakchi asl
    • 1
  • Ali akbar Mozafari
    • 1
    Email author
  • Nasser Ghaderi
    • 1
  1. 1.Department of Horticultural SciencesFaculty of Agriculture, University of KurdistanSanandajIran

Personalised recommendations