The in vitro propagation system of Citrus × latifolia (Yu. Tanaka) Yu. Tanaka (Rutaceae) affects the growth and depletion of nutriments
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Among citrus fruits, Citrus × latifolia (Yu. Tanaka) Yu. Tanaka stands out given its diverse uses, and there is a great demand for plants of high phytosanitary and genetic quality. In the present study, the in vitro micropropagation process of C. latifolia was studied in RITA®-type bioreactor, semisolid medium, and partial immersion with liquid culture medium. Explants that were 2 mo of age were used and apices of 0.5 cm were extracted and placed in each culture system. In all the systems, MS (Murashige and Skoog) culture medium was used, supplemented with 6-benzylaminopurine (BAP) and kinetin (KIN). After 30 d, the number of shoots, the size of the shoots, the number of leaves, and the depletion of nutrients from the culture medium were analyzed. With the data obtained, ANOVA and Tukey’s mean comparison tests were performed. The RITA® bioreactors, with the addition of 40 mL of medium per explant in a 4-h-interval immersion, facilitated the production of the greatest number of shoots compared to the other culture systems, and also resulted in the highest levels in medium nutrient depletion.
KeywordsPersian lemon Bioreactors RITA® Nutrients Shoots
The authors wish to thank the Comité Ejecutivo Nacional Sistema Producto Limón Persa A. C. Cuitláhuac, Veracruz, México, for their support.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Alcántar GG, Sandoval MV (1999) Manual de análisis químico de tejido vegetal. Publicación especial. Núm. 10. SMCS. Chapingo, Texcoco, Estado de México. 150 pGoogle Scholar
- Clostre G, Suni M (2007) Efecto del nitrógeno, fósforo y potasio del medio de cultivo en el rendimiento y valor nutritivo de Lemna gibba L. (Lemnaceae). Rev Peru Biol 13:231–235Google Scholar
- El-Sherif NA (2017) Impact of plant tissue culture on agricultural sustainability. In: Barceló D, Kostianoy AG (eds) The handbook of environmental chemistry. Springer, BerlinGoogle Scholar
- Food and Agricultural Organization (2017) Citrus fruit - fresh and processed statistical bulletin 2016. Food and agriculture organization of the united nations, RomeGoogle Scholar
- Jaiswal DK, Prakash Verma J, Prakash S, Meena VS, Meena RS (2016) Potassium as an important plant nutrient in sustainable agriculture: a state of the art. In: Meena VS, Maurya BR, Verma JP, Meena RS (eds) Potassium solubilizing microorganisms for sustainable agriculture. Springer, New Delhi, pp 21–29CrossRefGoogle Scholar
- Jin S, Park J, Park S, Lee D, Yun S (2017) Production of citrus plants from ovule cell culture and verification of CTV-free plants. Hortic Sci Technol 35:121–130Google Scholar
- Krapp A (2015) Plant nitrogen assimilation and its regulation: a complex puzzle with missing pieces. Plant Biol 25:115–122Google Scholar
- Posada-Pérez L, Montesinos YP, Guerra DG, Daniels D, Gómez-Kosky R (2017) Complete germination of papaya (Carica papaya L. cv. ‘Maradol Roja’) somatic embryos using temporary immersion system type RITA® and phloroglucinol in semisolid culture medium. In Vitro Cell Dev Biol-Plant 53:505–513CrossRefGoogle Scholar
- Servicio de Información Agroalimentaria y Pesquera (2016) http://www.siap.sagarpa.gob.mx/ Accessed 20-03-2018
- Tallón CI, Córdoba F, Porras I, Pérez-Tornero O (2015) Efficient in vitro propagation and rooting of adult explants of citrus rootstocks. Acta Hortic 1065:649–656. https://doi.org/10.17660/ActaHortic.2015.1065.81
- Tallón CI, Porras I, Pérez-Tornero O (2013) High efficiency in vitro organogenesis from mature tissue explants of Citrus macrophylla and C. aurantium. In Vitro Cell Dev Biol-Plant 49:145–155Google Scholar
- Werner S, Maschke RW, Eibl D, Eibl R (2017) Bioreactor technology for sustainable production of plant cell-derived products. In: Pavlov A, Bley T (eds) Bioprocessing of plant in vitro systems, Reference series in phytochemistry. Springer, Basel, pp 1–20Google Scholar