Advertisement

Goji berry (Lycium barbarum L.) in vitro multiplication improved by light-emitting diodes (LEDs) and 6-benzylaminopurine

  • Débora de Oliveira PrudenteEmail author
  • Lucas Batista de Souza
  • Renato Paiva
  • Débora Domiciano
  • Pollyanna Aparecida de Carvalho
  • Fernanda Carlota Nery
Plant Development/Regeneration
  • 135 Downloads

Abstract

In Brazil, Lycium barbarum L. (goji berry) is an agronomically valuable, imported, commercialized plant. The present study evaluated the effects of cytokinins and light sources on optimization of in vitro multiplication of goji berry by axillary bud proliferation. Nodal explants were excised from plants 90 d after in vitro germination and cultured on Murashige and Skoog (MS) medium containing 30 g L−1 sucrose; 7 g L−1 agar-agar; and 0, 2, 4, 8, or 16 μM 6-benzylaminopurine (BAP), 6-furfurylaminopurine (KIN), or thidiazuron (TDZ). After 60 days, regenerated lateral buds and shoot lengths were evaluated. The regenerated shoots were inoculated in MS supplemented with 0.0, 2.5, 5.0, 10.0, or 20.0 μM BAP under white fluorescent lamps or mixed treatment of red/blue (RB) light-emitting diode (LED) lamps. After 60 days in vitro cultivation, the number of formed shoots, leaf number, and shoot length were determined. Subsequently, the shoots were separated and acclimatized in a greenhouse for 30 d and the percentage of survival was evaluated. High regeneration and shoot numbers during lateral bud regeneration occurred with MS medium plus 4 μM BAP. The estimated optimum concentration of 11.56 μM BAP under RB LED lamp resulted in a high shoot number (8.15), with more average leaves per plant (9.2) at 5 μM BAP, and longer shoots (12.34 cm) at 12.42 μM BAP. Acclimatization was successful with 100% survival of ex vitro plants. An efficient in vitro multiplication protocol of an economically important species, goji berry, was developed to facilitate large-scale commercialization of this species.

Keywords

Goji berry (Lycium barbarum L.) micropropagation Cytokinins Light sources Medicinal species Solanaceae 

Notes

Funding information

The authors are grateful to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Brasília, DF—Brazil), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG; Belo Horizonte, MG—Brazil), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES; Brasília, DF—Brazil) for financial support.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

References

  1. Al-Khayri JM, Naik PM (2017) Date palm micropropagation: advances and applications. Ciênc agrotec 41:347–358CrossRefGoogle Scholar
  2. Al-Mayahi AMW (2016) Effect of red and blue light emitting diodes “CRB-LED” on in vitro organogenesis of date palm (Phoenix dactylifera L.) cv. Alshakr. World J Microbiol Biotechnol 32:160–168CrossRefGoogle Scholar
  3. Andrade HB, Braga AF, Bertolucci SKV, Hsie BS, Silva ST, Pinto JEBP (2015) Effect of plant growth regulators, light intensity and LED on growth and volatile compound of Hyptis suaveolens (L.) Poit in vitro plantlets. Acta Hortic 1155:277–284Google Scholar
  4. Batista DS, Castro KM, Silva AR, Teixeira ML, Sales TA, Soares LI, Otoni WC (2016) Light quality affects in vitro growth and essential oil profile in Lippia alba (Verbenaceae). In Vitro Cell Dev Biol - Plant 52:276–282CrossRefGoogle Scholar
  5. Chen CC, Agrawal DC, Lee MR, Lee RJ, Kuo CL, Wu CR, Chang HC (2016) Influence of LED light spectra on in vitro somatic embryogenesis and LC-MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb. Bot Stud 57:1–9Google Scholar
  6. Cioć M, Szewczyk A, Żupnik M, Kalisz A, Pawłowska B (2018) LED lighting affects plant growth, morphogenesis and phytochemical contents of Myrtus communis L. in vitro. Plant Cell Tissue Organ Cult 132:433–447CrossRefGoogle Scholar
  7. Croser JS, Pazos-Navarro M, Bennett RG, Tschirren S, Edwards K, Erskine W, Ribalta FM (2016) Time to flowering of temperate pulses in vivo and generation turnover in vivo–in vitro of narrow-leaf lupin accelerated by low red to far-red ratio and high intensity in the far-red region. Plant Cell Tissue Organ Cult 127:591–599CrossRefGoogle Scholar
  8. Dănăilă-Guidea S-M, Dobrinoiu R-V, Vişan L, Toma RC (2015) Protocol for efficient in vitro multiplication of Lycium barbarum L. (goji) by direct organogenesis. Sci Bull Series F Biotechnol 19:34–38Google Scholar
  9. Farhadi N, Panahandeh J, Azar AM, Salte SA (2017) Effects of explant type, growth regulators and light intensity on callus induction and plant regeneration in four ecotypes of Persian shallot (Allium hirtifolium). Sci Hortic 218:80–86CrossRefGoogle Scholar
  10. Ferreira DF (2014) Sisvar: a guide for its bootstrap procedures in multiple comparisons. Ciênc agrotec 38:109–112CrossRefGoogle Scholar
  11. Ferreira LT, Silva MMD, Ulisses C, Camara TR, Willadino L (2017) Using LED lighting in somatic embryogenesis and micropropagation of an elite sugarcane variety and its effect on redox metabolism during acclimatization. Plant Cell Tissue Organ Cult 128:211–221CrossRefGoogle Scholar
  12. Fira A, Joshee N, Cristea V, Simu M, Hârța M, Pamfil D, Clapa D (2016) Optimization of micropropagation protocol for goji berry (Lycium barbarum L.). Bull UASVM Horticulture 3:141–150Google Scholar
  13. Fratianni A, Niro S, Alam MDR, Cinquanta L, Di Matteo M, Adiletta G, Panfili G (2018) Effect of a physical pre-treatment and drying on carotenoids of goji berries (Lycium barbarum L.). LWT 92:318–323CrossRefGoogle Scholar
  14. Gupta SD, Agarwal A (2017) Influence of LED lighting on in vitro plant regeneration and associated cellular redox balance. In: Gupta SD (ed) Light emitting diodes for agriculture. Springer, Singapore, pp 273–303CrossRefGoogle Scholar
  15. Gupta SD, Jatothu B (2013) Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotech Rep 7:211–220CrossRefGoogle Scholar
  16. Hu Z, Hu Y, Gao HH, Guan XQ, Zhuang DH (2008) Callus production, somatic embryogenesis and plant regeneration of Lycium barbarum root explants. Biol Plant 52:93–96CrossRefGoogle Scholar
  17. Hu Z, Wu YR, Li W, Gao HH (2006) Factors affecting Agrobacterium tumefaciens-mediated genetic transformation of Lycium barbarum L. In Vitro Cell Dev Biol - Plant 42:461–466CrossRefGoogle Scholar
  18. Hung CD, Hong CH, Kim SK, Lee KH, Park JY, Nam MW, Lee HI (2016) LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry (Vaccinium corymbosum L.). Acta Physiol Plant 38:1–9CrossRefGoogle Scholar
  19. Khan N, Ahmed M, Hafiz I, Abbasi N, Ejaz S, Anjum M (2015) Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes. OENO One 49:37–45CrossRefGoogle Scholar
  20. Kulczyński B, Gramza-Michałowska A (2016) Goji berry (Lycium barbarum): composition and health effects–a review. Pol J Food Nutr Sci 66:67–76CrossRefGoogle Scholar
  21. Lam SC, Luo Z, Wu DT, Cheong KL, Hu DJ, Xia ZM, Li SP (2016) Comparison and characterization of compounds with antioxidant activity in Lycium barbarum using high-performance thin layer chromatography coupled with DPPH bioautography and tandem mass spectrometry. J Food Sci 81:1378–1384CrossRefGoogle Scholar
  22. Li CX, Xu ZG, Dong RQ, Chang SX, Wang LZ, Khalil-Ur-Rehman M, Tao JM (2017) An RNA-seq analysis of grape plantlets grown in vitro reveals different responses to blue, green, red LED light, and white fluorescent light. Front Plant Sci 8:1–16Google Scholar
  23. Li XM, Ma YL, Liu XJ (2007) Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice. J Ethnopharmacol 111:504–511CrossRefGoogle Scholar
  24. Lin KH, Huang MY, Huang WD, Hsu MH, Yang ZW, Yang CM (2013) The effects of red, blue, and white light-emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata). Sci Hortic 150:86–91CrossRefGoogle Scholar
  25. Manivannan A, Soundararajan P, Park YG, We H, Kim S, Jeong BR (2017) Blue and red light-emitting diodes improve the growth and physiology of in vitro-grown carnations ‘Green Beauty’ and ‘Purple Beauty. Hortic Environ Biotechnol 58:12–20CrossRefGoogle Scholar
  26. Ming M, Guanhua L, Zhanhai Y, Guang C, Xuan Z (2009) Effect of the Lycium barbarum polysaccharides administration on blood lipid metabolism and oxidative stress of mice fed high-fat diet in vivo. Food Chem 113:872–877CrossRefGoogle Scholar
  27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  28. Osman NI, Awal A, Sidik NJ, Abdullah S (2013a) Callus induction and somatic embryogenesis from leaf and nodal explants of Lycium barbarum L. (goji). Biotech 12:36–45CrossRefGoogle Scholar
  29. Osman NI, Awal A, Sidik NJ, Abdullah S (2013b) In vitro regeneration and antioxidant properties of Lycium barbarum L. (goji). J Teknol (Sciences & Engineering) 62:35–38Google Scholar
  30. Pai PG, Habeeba PU, Ullal S, Shoeb PA, Pradeepti MS, Ramya K (2013) Evaluation of hypolipidemic effects of Lycium barbarum (goji berry) in a murine model. J Nat Remedies 13:4–8Google Scholar
  31. Ratushnyak YI, Piven NM, Rudas VA (1989) Protoplast culture and plant regeneration in Lycium barbarum L. Plant Cell Tissue Organ Cult 17:183–190CrossRefGoogle Scholar
  32. Tonkha OL, Dzyazko YS (2014) Soils and plant roots. In: Volfkovich YM, Filippov AN, Bagotsky VS (eds) Structural properties of porous materials and powders used in different fields of science and technology. Springer, London, UK, pp 221–249Google Scholar
  33. Trigiano RN, Gray DJ (2016) Plant tissue culture, development, and biotechnology. CRC Press, Boca Raton, FLCrossRefGoogle Scholar
  34. Tudor V, Asănică A, Teodorescu RI, Gidea M, Tănăsescu C, Tudor AD, Țiu JV (2017) Germination capacity of some Lycium barbarum L. and Lycium chinense Mill. biotypes seeds. Rom Biotechnol Lett 22:12191–12196Google Scholar
  35. Wu SJ, Ng LT, Lin CC (2004) Antioxidant activities of some common ingredients of traditional Chinese medicine, Angelica sinensis, Lycium barbarum and Poriacocos. Phytother Res 18:1008–1012CrossRefGoogle Scholar
  36. Xin T, Yao H, Gao H, Zhou X, Ma X, Xu C, Song J (2013) Super food Lycium barbarum (Solanaceae) traceability via an internal transcribed spacer 2 barcode. Food Res Int 54:1699–1704CrossRefGoogle Scholar
  37. Youlong C, Qing L, Xiyan Z, Xiaoying L (2008) Effects of different culture conditions on vitrification of Lycium barbarum L. plantlets in tissue culture. J Agric Sci Technol 9:30–32Google Scholar

Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Universidade Federal de Lavras (UFLA)LavrasBrazil
  2. 2.Universidade Federal de São João Del Rei (UFSJ)São João Del ReiBrazil

Personalised recommendations