Goji berry (Lycium barbarum L.) in vitro multiplication improved by light-emitting diodes (LEDs) and 6-benzylaminopurine

  • Débora de Oliveira PrudenteEmail author
  • Lucas Batista de Souza
  • Renato Paiva
  • Débora Domiciano
  • Pollyanna Aparecida de Carvalho
  • Fernanda Carlota Nery
Plant Development/Regeneration


In Brazil, Lycium barbarum L. (goji berry) is an agronomically valuable, imported, commercialized plant. The present study evaluated the effects of cytokinins and light sources on optimization of in vitro multiplication of goji berry by axillary bud proliferation. Nodal explants were excised from plants 90 d after in vitro germination and cultured on Murashige and Skoog (MS) medium containing 30 g L−1 sucrose; 7 g L−1 agar-agar; and 0, 2, 4, 8, or 16 μM 6-benzylaminopurine (BAP), 6-furfurylaminopurine (KIN), or thidiazuron (TDZ). After 60 days, regenerated lateral buds and shoot lengths were evaluated. The regenerated shoots were inoculated in MS supplemented with 0.0, 2.5, 5.0, 10.0, or 20.0 μM BAP under white fluorescent lamps or mixed treatment of red/blue (RB) light-emitting diode (LED) lamps. After 60 days in vitro cultivation, the number of formed shoots, leaf number, and shoot length were determined. Subsequently, the shoots were separated and acclimatized in a greenhouse for 30 d and the percentage of survival was evaluated. High regeneration and shoot numbers during lateral bud regeneration occurred with MS medium plus 4 μM BAP. The estimated optimum concentration of 11.56 μM BAP under RB LED lamp resulted in a high shoot number (8.15), with more average leaves per plant (9.2) at 5 μM BAP, and longer shoots (12.34 cm) at 12.42 μM BAP. Acclimatization was successful with 100% survival of ex vitro plants. An efficient in vitro multiplication protocol of an economically important species, goji berry, was developed to facilitate large-scale commercialization of this species.


Goji berry (Lycium barbarum L.) micropropagation Cytokinins Light sources Medicinal species Solanaceae 


Funding information

The authors are grateful to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Brasília, DF—Brazil), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG; Belo Horizonte, MG—Brazil), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES; Brasília, DF—Brazil) for financial support.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Universidade Federal de Lavras (UFLA)LavrasBrazil
  2. 2.Universidade Federal de São João Del Rei (UFSJ)São João Del ReiBrazil

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