Advertisement

In Vitro Cellular & Developmental Biology - Plant

, Volume 55, Issue 1, pp 121–131 | Cite as

Peculiarities of calcium and iron effects on some wild terrestrial orchids in vitro compared to in vivo

  • Gunta JakobsoneEmail author
  • Anita Osvalde
Plant Tissue Culture
  • 45 Downloads

Abstract

There are 32 species from the family Orchidaceae in Latvia, and 26 are rare and endangered, and their preservation in an in vitro bank is vital. The death of in vitro grown wild terrestrial orchids is mainly caused by the release of phenolic compounds from root tissues. As mineral nutrients form a significant component of culture media, a hypothesis was advanced that increased doses of both Ca and Fe could prevent phenol oxidation and improve micropropagation. Liparis loeselii (L.) Rich. and Gymnadenia conopsea (L.) R. Br. calcicole plants, and Dactylorhiza russowii (Klinge) Holub, a non-calcicole plant, were used as model species. Modification of the culture media with increased Ca gluconate monohydrate concentrations significantly improved the quality of L. loeselii and D. russowii plants, especially in the presence of elevated levels of ferric citrate. There was no benefit of Ca for G. conopsea, except in combination with the highest level of ferric citrate tested. The results revealed species-specific stimulatory or inhibitory impacts of changes in the pH of culture media on orchid plantlet quality. These findings demonstrate the crucial role of iron to prevent necrosis. The results indicated that for species with relatively higher adaptation potential to growth in habitats with different pH levels (L. loeselii and D. russowii), the shoot quality in vitro was better if the Ca to Fe ratio in the culture medium was 2:1. Despite the fact that G. conopsea is a calcicole species, the optimal Ca to Fe ratio in the culture medium was 1:1.

Keywords

Liparis loeselii Gymnadenia conopsea Dactylorhiza russowii pH effect 

Notes

Acknowledgements

Greatest thanks to Andrejs Svilāns, Director of the National Botanic Garden for support of this research. We would like to thank PhD Daina Roze, researcher in the Department of Dendrology of National Botanic Garden for collaboration, and Anita Dūda, laboratory assistant in the Department of Plant Eco-Physiology of National Botanic Garden of Latvia for technical assistance.

References

  1. Abadía J, López-Millán AF, Rombolà A, Abadía A (2002) Organic acids and Fe deficiency: a review. Plant Soil 241:75–86CrossRefGoogle Scholar
  2. Abadía J, Monge E, Montañés L, Heras L (1984) Extraction of iron from plant leaves by Fe (II) chelators. J Plant Nutr 7:777–784CrossRefGoogle Scholar
  3. Ammari T, Mengel K (2006) Total soluble Fe in soil solutions of chemically different soils. Geoderma 136:876–885CrossRefGoogle Scholar
  4. Andrus RE (1986) Some aspects of Sphagnum ecology. Can J Bot 64:416–426CrossRefGoogle Scholar
  5. Andrušaitis G (ed.) (2003) Red data book of Latvia. Rare and threatened plants and animals. Vol. 3 Vascular plants. Institute of Biology of University of Latvia, Riga, LatviaGoogle Scholar
  6. Anonymous (1996) Analytical methods for atomic absorption spectrometry. Perkin-Elmer Corporation, Waltham, Massachusetts http://www1.lasalle.edu/~prushan/Intrumental%20Analysis_files/AA-Perkin%20Elmer%20guide%20to%20all!.pdf
  7. Auniņš A (ed.) (2013) EU protected habitats in Latvia. Detection manual. Revised edition 2. Latvian Fund for Nature, Ministry of Environmental Protection and Regional Development, Riga, Latvia (in Latvian)Google Scholar
  8. Barber SA (1995) Soil nutrient bioavailability: a mechanistic approach. Ed 2. John Wiley & Sons, New York, New YorkGoogle Scholar
  9. Boxma R (1972) Bicarbonate as the most important soil factor in lime-induced chlorosis in the Netherlands. Plant Soil 37:233–243CrossRefGoogle Scholar
  10. Brown JC (1978) Mechanism of iron uptake by plants. Plant Cell Environ 1:249–257CrossRefGoogle Scholar
  11. Brown JC, Jolley VD (1998) Comparative evaluation of iron management in plants, animals, and humans: possible mechanisms for iron use by cancer cells independent of their host. J Plant Nutr 21:1529–1538CrossRefGoogle Scholar
  12. Burstrom H (1963) Growth regulation by metals and chelates. Adv Bot Res 1:73–100CrossRefGoogle Scholar
  13. Catling PM (1980) Rain-assisted autogamy in Liparis loeselii (L.) L.C.Rich. (Orchidaceae). Bull Torrey Bot Club 107:525–529CrossRefGoogle Scholar
  14. Cepurīte B (2005) Vascular flora of Latvia 7: Orchidaceae (Orchid family). University of Latvia, Riga, Latvia (in Latvian)Google Scholar
  15. De Silva DLR, Hetherington AM, Mansfield TA (1996) Where does all the calcium go? Evidence of an important regulatory role for trichomes in two calcicoles. Plant Cell Environ 19:880–886Google Scholar
  16. Ellenberg H (1996) Vegetation Mitteleuropas mit den Alpen. 5. Auflag. Verlag Eugen Ulmer, Stuttgart, GermanyGoogle Scholar
  17. Ellenberg H, Weber HE., Düll R, Wirth W, Werner W, Paulissen D (1992) Zeigerwerte von Pflanzen in Mitteleuropa (2nd ed.) [Indicator values for plant in Central Europe]. Scr Geobot 18:1–258Google Scholar
  18. Fageria NK (2009) The use of nutrients in crop plants. CRH press, New York, New YorkGoogle Scholar
  19. Fast K (1974) Die Orchidee. 25:125–129 (Cited in Rasmussen H (1995) Terrestrial orchids from seed to mycotrophic plant. Cambridge Univ Press, Cambridge, United Kingdom)Google Scholar
  20. George EF, Hall MA, De Klerk GJ (2008) The components of plant tissue culture media I: macro- and micro-nutrients. In: George EF, Hall MA, De Klerk GJ. (eds.) Plant propagation by tissue culture. 3rd Edition. Springer, Rotterdam, The Netherlands, pp. 65–113Google Scholar
  21. GSPC (2010) Global strategy for plant conservation (http://www.plants2010.org/)
  22. Jakobsone G (2008) Morphogenesis of wild orchid Dactylorhiza fuchsii in tissue culture. Acta Univ Latv, ser Biol 745:17–23Google Scholar
  23. Jakobsone G (2009) Germination and development of some terrestrial orchids in vitro. (proceedings of the third international symposium on acclimatization and establishment of micropropagated plants), Portugal. Acta Hortic 812:533–537CrossRefGoogle Scholar
  24. Jakobsone G, Belogrudova I, Megre D (2010) Dactylorhiza fuchsii as model object in in vitro culture study for development of terrestrial orchids. Acta Biol Univ Daugavp., suppl. 2:41–48 ISSN:1407–8953Google Scholar
  25. Jakobsone G, Belogrudova I, Roze D, Megre D (2012) Dactylorhiza baltica in vitro and in vivo. Acta Biol Univ Daugavp 12:151–165Google Scholar
  26. Jin CW, You GY, He YF, Tang CX, Wu P, Zheng SJ (2007) Iron-deficiency-induced secretion of phenolics facilitates the reutilization of root apoplastic iron in red clover (Trifolium pratense L.). Plant Physiol 144:278–285CrossRefPubMedPubMedCentralGoogle Scholar
  27. Kim SA, Guerinot ML (2007) Mining iron: Iron uptake and transport in plants. FEBS Lett 581:2273–2280CrossRefPubMedGoogle Scholar
  28. Kumari S, Elancheran R, Kotoky J, Devi R (2016) Rapid screening and identification of phenolic antioxidants in Hydrocotyle sibthorpioides lam by UPLC–ESI-MS/MS. Food Chem 203:521–529CrossRefPubMedGoogle Scholar
  29. Lambers H, Chapin III FS, Pons TL (2008) Plant physiological ecology. Springer, New York, New YorkGoogle Scholar
  30. Laukkanen H, Häggman H, Kontunen-Soppelaa S, Hohtolaa A (1999) Tissue browning of in vitro cultures of scots pine: role of peroxidase and polyphenol oxidase. Physiol Plant 106:337–343CrossRefGoogle Scholar
  31. Marschner H (1995) Mineral nutrition in higher plants. In: Academic press. United Kingdom, LondonGoogle Scholar
  32. Mehrotra SC, Gupta P (1990) Reduction of iron by leaf extracts and its significance for the assay of Fe (II) iron in plants. Plant Physiol 93:1017–1020CrossRefPubMedPubMedCentralGoogle Scholar
  33. Megre D, Roze D, Dokane K, Jakobsone G, Karlovska A (2018) Survival of an endangered orchid Liparis loeselii in habitats with different level fluctuations. Pol J Ecol 66:126–138CrossRefGoogle Scholar
  34. Mengel K (1994) Iron availability on plant tissues – iron chlorosis in calcareous soils. Plant Soil 165:275–283CrossRefGoogle Scholar
  35. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  36. Norstog K (1973) New synthetic medium for the culture of premature barley embryos. In Vitro Cell Dev Biol-Plant 8:307–308CrossRefGoogle Scholar
  37. North J, Ndakidemi P, Laubscher C (2012) Effects of antioxidants, plant growth regulators and wounding on phenolic compound excretion during micropropagation of Strelitzia Reginae. Int J Phys Sci 7:638–646CrossRefGoogle Scholar
  38. Page AL, Miller RH, Keeney DR (eds.) (1982) Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties. American Society of Agronomy, Soil Society of America, Madison, WisconsinGoogle Scholar
  39. Pan MJ, van Staden J (1998) The use of charcoal in in vitro culture – a review. Plant Growth Regul 26:155–163CrossRefGoogle Scholar
  40. Pikner T (2012) Taxonomic diversity of Dactylorhiza on Saaremaa. J Hardy Orchid Soc 9:128–143Google Scholar
  41. Pillon Y, Qamaruz-Zaman F, Fay MF, Hendoux F, Piquot Y (2007) Genetic diversity and ecological differentiation in the endangered fen orchid (Liparis loeselii). Conserv Genet 8:177–184CrossRefGoogle Scholar
  42. Pritchard J (1994) The control of cell expansion in roots. New Phytol 127:3–26CrossRefGoogle Scholar
  43. Rasmussen HN (1995) Terrestrial orchids from seed to mycotrophic plant. Cambridge University press, New York, New YorkGoogle Scholar
  44. Remm K, Linder M, Remm L (2009) Relative density of finds for assessing similarity-based maps of orchid occurrence. Ecol Model 220:294–309CrossRefGoogle Scholar
  45. Rinkis GJ, Ramane HK, Kunickaya TA (1987) Methods of soil and plant analysis. Zinatne, Riga, Latvia (in Russian)Google Scholar
  46. Romera FJ, Alcántara E, de la Guardia MD (1991) Characterization of the tolerance to iron chlorosis in different peach rootstocks grown in nutrient solution. I. Effect of bicarbonate and phosphate. Plant Soil 130:115–119CrossRefGoogle Scholar
  47. Roze D (2015) Impact of the ecological factors on viability of populations Liparis loeselii (L.) Rich. in Latvia. The Doctoral Thesis, Daugavpils University, Daugavpils, Latvia https://biblio.du.lv/autortiesibas.asp?url=http://dnl.biblio.du.lv/promocijas/DU_2015_daina_roze_promdarbs.pdf
  48. Roze D, Megre D, Jakobsone G (2015) Study on micro-habitats for understanding the ecology and management requirements of Liparis loeselii populations in Latvia. Latvijas Veģetācija 24:5−28 (in Latvian)Google Scholar
  49. Salmiņa L (2009) Limnogenous bog vegetation in Latvia. Latvijas Veģetācija 19:1–181 (in Latvian) http://www.silava.lv/userfiles/file/Latvijas%20Vegetacija/Lat_Veg_19_2009.pdf Google Scholar
  50. Tullock J (2005) Growing hardy orchids. Timber press, Inc., Portland, OregonGoogle Scholar
  51. Tyler G, Ström L (1995) Differing organic acid exudation pattern explains calcifuge and acidifuge behaviour of plants. Ann Bot 75:75–78CrossRefPubMedPubMedCentralGoogle Scholar
  52. Zohlen A, Tyler G (2000) Immobilization of tissue iron on calcareous soil: differences between calcicole and calcifuge plants. Oikos 89:95–106CrossRefGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Department of Plant Eco-PhysiologyNational Botanic GardenSalaspilsLatvia
  2. 2.Laboratory of Plant Mineral NutritionInstitute of Biology University of LatviaRigaLatvia

Personalised recommendations