In vitro growth profile and comparative leaf anatomy of the C3–C4 intermediate plant Mollugo nudicaulis Lam.
- 72 Downloads
Mollugo nudicaulis Lam., commonly known as John’s folly or naked-stem carpetweed, is an ephemeral species of tropical regions. The plant is ideal to study the eco-physiological adaptations of C3–C4 intermediate plants. In the present report, in vitro growth profiling of the plant and comparative leaf anatomy under in vitro and ex vitro conditions were studied. In vitro propagation of the plant was carried out on Murashige and Skoog (MS) basal medium augmented with additives and solidified with 0.8% (w/v) agar-agar or 0.16% (w/v) Phytagel™. The concentration of plant growth regulators (PGRs) in the basal medium was optimized for callus induction, callus proliferation, shoot regeneration, and in vitro rooting. The optimum callus induction was obtained from M. nudicaulis seedling hypocotyls. The highest regeneration induction of about 88% or nearly 41 shoots with about 142 leaves per culture vessel was observed from friable callus on MS basal medium solidified with Phytagel™ and containing 4.44 μM 6-benzylaminopurine, 4.65 μM kinetin, 2.69 μM naphthaleneacetic acid, and 0.91 μM thidiazuron. In leaf anatomy, differences related to photosynthetic tissue organization were observed in leaves of in vitro and ex vitro plants, which indicated that changes in the environment affected the anatomy of subsequent leaves in plants. This is the first report of an efficient micropropagation protocol for M. nudicaulis, using an indirect organogenesis method. Efforts were made to optimize the concentrations of various PGRs and organic compounds for in vitro growth of regenerated shoots.
KeywordsMicropropagation Tropical C3–C4 intermediate Phytagel Gibberellic acid Aeroponics
Authors are thankful to the University Grant Commission (UGC), New Delhi, for providing Special Assistance Program (SAP) in the form of Centre of Advanced Study (CAS) to the Department of Botany, Jai Narain Vyas University, and Jodhpur.
- Bhandari MM (1990) Flora of the Indian desert. MPS Repros, Jodhpur, pp 159–160Google Scholar
- Chaâbani G, Tabart J, Kevers C, Dommes J, Khan MI, Zaoui S, Chebchoub L, Lachaâl M, Karray BN (2015) Effects of 2,4-Dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. Aronia. Acta Physiol Plant 37:16CrossRefGoogle Scholar
- Gomez KA, Gomez AA (1984) Single factor experiments. In: Statistical analysis procedure of agricultural research. John Wiley and sons, New York, pp 7–29Google Scholar
- Mohlakola EM, Cheng C, Lin Y, Guo R, Min KT, Chen Y, Lai Z (2017) Effects of 2, 4-Dichlorophenoxy acetic acid and light on growth of Gerbera (Gerbera jamesonii cv. Daxueju) callus. J Agr Sci Tech 18:385–393Google Scholar
- Nagesh KS, Shanthamma C (2011) Micropropagation and antioxidant activity of Mollugo nudicaulis lam. J Med Plants Res 5:895–902Google Scholar
- Patel AK, Lodha D, Ram K, Shekhawat S, Shekhawat NS (2016) Evaluation of physiochemical factors affecting high frequency plant regeneration of Blyttia spiralis (Forssk.) D.V. Field & J.R.I. wood [synonym: Pentatropis spiralis (Forssk.) Decne.], a threatened climber of medicinal values. In Vitro Cell Dev Biol-Plant 52:10–19CrossRefGoogle Scholar
- The International Plant Names Index (2012) http://ipni.org names: 363731–1. Cited on 15 Jan 2018
- Vincent MA (2003) Molluginaceae Rafinesque: Flora of North America Editorial Committee. Flora of North America, volume 4: Magnoliophyta: Caryophyllidae, Part 1, Oxford Univ. Press, Oxford, UKGoogle Scholar