Cell suspension culture of Rhizoma zedoariae in a two-stage perfusion bioreactor system for β-elemene production

  • Gui Rong Wang
  • Hui WangEmail author
Plant Tissue Culture


We presented a two-stage combined bioreactor system consisting of a stir-tank and an airlift column, and challenged with Rhizoma zedoariae cell suspensions for β-elemene production. Two-stage culture was initiated when the cell concentration in both vessels was maintained at an appropriate density. The cells were proliferated in stirred-tank with the maximal growth rate of 0.17 d−1 to present enough cells for β-elemene synthesis. In the airlift column, continuous cell separation from culture medium was achieved by using a cell retention device based on centrifugal and gravity settling when the system was performed in perfusion mode. The results indicated that additives can efficiently promote the accumulation of β-elemene in R. zedoariae cells. In addition, the β-elemene content showed higher levels in cell lines of overexpressing 3-hydroxy-3-methylglutaryl coenzyme-A reductase, Farnesyldi phosphate synthase, and ST02C genes.


β-Elemene Bioreactor Cell culture Rhizoma zedoariae Secondary metabolism 



This work was supported by the Shanghai natural science fund (No.BS150004).


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© The Society for In Vitro Biology 2018

Authors and Affiliations

  1. 1.School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghaiChina
  2. 2.School of Health Science and NursingShanghai Sipo PolytechnicShanghaiChina

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