In vitro preservation and micropropagation of Oreocharis mileense (W.T. Wang) M. Möller & A. Weber (Gesneriaceae) through shoot organogenesis
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Oreocharis mileense (W.T. Wang) M. Möller & A. Weber is endemic to China and was considered to be extinct because it had not been seen in the wild since the first collection in 1906. In 2006, the species was rediscovered in Shilin County, Yunnan Province. Oreocharis mileense was considered critically endangered for its narrow geographic range and extremely small population. An efficient method to preserve plant germplasm by in vitro culturing of O. mileense has not been reported. In this study, an orthogonal array with three factors (6-benzyladenine, BA; α-naphthaleneacetic acid, NAA; and sucrose), at four levels was performed, and shoot induction as well as shoot proliferation were recorded. The results were analyzed to determine the most significant components and the optimum combination for micropropagation of O. mileense. The results showed that: (1) organogenesis was easily induced by different combinations of plant-growth regulators and sucrose; (2) NAA and sucrose had the most significant effect on shoot induction and shoot multiplication, and (3) the optimum induction and proliferation media were 0.5 mg L−1 BA + 0.2 mg L−1 NAA + 30 g L−1 sucrose and 1 mg L−1 BA + 0.1 mg L−1 NAA + 30 g L−1 sucrose, respectively.
KeywordsOreocharis mileense Rediscovery Critically endangered In vitro preservation Micropropagation
The authors in particular wish to thank Professor David E. Boufford for editing the English.
This study was supported by the Ministry of Science and Technology of China (2005DKA21006, 2012FY110300), the National Natural Science Foundation of China (31761143001, 31870316, 31070288, 31560063), the Ministry of Education of China (yldxxk201819, 2015MDTD16C, B08044), and the Key Laboratory of Ethnomedicine (Minzu University of China) of the Ministry of Education of China (KLEM-ZZ201806).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Gao Q, Xu WB (2011) Paraisometrum W. T. Wang, a newly recorded genus of Gesneriaceae from Guizhou, China. Acta Botan Boreali-Occiden Sin 31:858–860 (In Chinese)Google Scholar
- Li J, Xing Q, Chen WL, Guo DH, Shi L (2009) Tissue culture and rapid propagation of Chirita medica D. Fang ex W.T. Wang. Propag Ornam Plant 9:97–101Google Scholar
- Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Int Plant Propagators Soc 30:421–427Google Scholar
- Shui YM (2006) Botanists rediscover a plant thought to be extinct. Bull Chin Acad Sci 20:206–207 (In Chinese)Google Scholar
- Shui YM, Cai J (2007) “100-years-lost” plant re-emerges in Yunnan, China. Samara 12:3Google Scholar
- Taha RM, Daud N, Hasbullah NA, Awal A (2009) Somatic embryogenesis and production of artificial seeds in Sintpaulia ionantha Weddl. Acta Hotic 829:331–336Google Scholar
- Tan Y, Wang Z, Sui XY, Hu GW, Motley T, Long CL (2011) The systematic placement of the monotypic genus Paraisometrum (Gesneriaceae) based on molecular and cytological data. Plant Divers 33:465–476Google Scholar
- Xiao LH, Yang G, Zhang LC, Yang XH, Zhao S, Ji ZZ, Zhou Q, Hu M, Wang Y, Chen M, Xu Y, Jin HJ, Xiao X, Hu GP, Bao F, Hu Y, Wan P, Li LG, Deng X, Kuang TY, Xiang CB, Zhu JK, Oliver MJ, He YK (2015) The resurrection genome of Boea hygrometrica: a blueprint for survival of dehydration. Proc Natl Acad Sci U S A 112:5833–5837CrossRefGoogle Scholar
- Xu WB, Pan B, Huang YS, Ye XX, Liu Y (2009b) Paraisometrum W. T. Wang, a newly recorded genus of Gesneriaceae from Guangxi, China. Guihaia 29:581–583 (In Chinese)Google Scholar