Advertisement

In Vitro Cellular & Developmental Biology - Plant

, Volume 54, Issue 6, pp 600–605 | Cite as

Quercetin and silver nitrate modulate organogenesis in Carissa carandas (L.)

  • Bhushan S. Bhadane
  • Vijay L. Maheshwari
  • Ravindra H. PatilEmail author
Micropropagation
  • 81 Downloads

Abstract

An in vitro organogenesis protocol for Carissa carandas L. was developed using an auxin transport inhibitor (quercetin) and silver nitrate (AgNO3), an inhibitor of ethylene action, in association with cytokinins in the culture medium. This protocol produced the maximum number of shoots from aseptic seedling-derived shoot apex explants of C. carandas. The highest rate of shoot multiplication was recorded on MS medium containing 2.0 mg L−1 6-benzylaminopurine; 0.5 mg L−1 kinetin, and 0.75 mg L−1 quercetin at after 4 wk of culture. Similar results were obtained when MS medium fortified with 2.0 mg L−1 BAP, 0.5 mg L−1 kinetin, and 1.5 mg L−1 AgNO3 was used. However, successful rooting was achieved on quarter strength MS medium with 0.5 mg L−1 indole-3-acetic acid. In this study, an inhibitor of auxin transport and ethylene action maximized shoot multiplication in medium fortified with cytokinins. The established rapid micropropagation method could be used to conserve elite genotypes of C. carandas.

Keywords

C. carandas Quercetin AgNO3 Cytokinins 

Notes

Funding information

Financial support was provided by SERB, New Delhi, and UGC, New Delhi.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

References

  1. Arora R, Bhojwani SS (1989) In vitro propagation and low temperature storage of Saussurea lappa CB Clarke—an endangered, medicinal plant. Plant Cell Rep 8:44–47CrossRefGoogle Scholar
  2. Begum S, Syed SA, Siddiqui BS, Sattar SA Choudhary MI (2013) Carandinol: first isohopane triterpene from the leaves of Carissa carandas L. and its cytotoxicity against cancer cell lines. Phytochem Lett 6:91–95CrossRefGoogle Scholar
  3. Bhadane BS, Patil RH (2016) Data on the cost-effective surface sterilization method for C. carandas (L.) seeds and callus induction from aseptic seedling. Data Brief 7:1551–1555CrossRefGoogle Scholar
  4. Bhadane BS, Patil RH (2017) Isolation, purification and characterization of antioxidative steroid derivative from methanolic extract of Carissa carandas (L.) leaves. Biocat and Agric Biotechnol 10:216–223Google Scholar
  5. Brown DE, Rashotte AM, Murphy AS, Normanly J, Tague BW, Peer WA, Muday GK (2001) Flavonoids act as negative regulators of auxin transport in vivo in Arabidopsis. Plant Physiol 126:524–535CrossRefGoogle Scholar
  6. Galipalli S, Patel NK, Prasanna K, Bhutani KK (2015) Activity-guided investigation of Carissa carandas (L.) roots for anti-inflammatory constituents. Nat Prod Res 29:1670–1672CrossRefGoogle Scholar
  7. George EF, Hall MA, De Klerk GJ (2008) Plant growth regulators II: cytokinins, their analogues and antagonists. In Plant propagation by tissue culture, George EF (Ed.). Springer Netherlands pp 205–226Google Scholar
  8. Hasmah SN, Bhatt A, Keng CL (2013) Micropropagation of Asam Karanda (Carissa carandas Linn). Pertanika J Trop Agric Sci 36:89–98Google Scholar
  9. Imran MA, Begum G, Sujatha K, Mallaiah B (2012) Effect of adenine sulphate (ads) with cytokinins on multiple shoot production in Carissa carandas (L.). Int J Pharm Bio Sci 3:473–480Google Scholar
  10. Itankar PR, Lokhande SJ, Verma PR, Arora SK, Sahu RA, Patil AT (2011) Antidiabetic potential of unripe Carissa carandas Linn. fruit extract. J Ethnopharmacol 135:430–433CrossRefGoogle Scholar
  11. Iyer CM, Dubash PJ (1993) Anthocyanin of karwand (Carissa carandas) and studies on its stability in model systems. J Food Sci Technol 30:246–248Google Scholar
  12. Jacobs M, Rubery PH (1988) Naturally occurring auxin transport regulators. Science 241:34CrossRefGoogle Scholar
  13. Kirtikar KR, Basu BD (1998) Data on medicinal plants and chemical constituents. Indian medicinal plants. VIMSAT publishers, Bangalore, pp 2532–2541Google Scholar
  14. Krishnan SS, Siril EA (2015) Enhanced in vitro shoot regeneration in Oldenlandia umbellata L. by using quercetin: a naturally occurring auxin-transport inhibitor. Proc Natl Acad Sci India Sect B Biol Sci 87:899–904CrossRefGoogle Scholar
  15. Lall S, Mandegaran Z, Roberts AV (2005) Shoot multiplication in cultures of mature Alnus glutinosa. Plant Cell Tissue Organ Cult 83:347–350CrossRefGoogle Scholar
  16. Mazri MA (2015) Role of cytokinins and physical state of the culture medium to improve in vitro shoot multiplication, rooting and acclimatization of date palm (Phoenix dactylifera). J Plant Biochem Biotechnol 24:268–275CrossRefGoogle Scholar
  17. Misra KK (2007) New karonda varieties from Pantnagar. Indian Hort 52:9Google Scholar
  18. Motwani SM, Pandey EP, Sonchhatra NP, Desai TR, Patel VL, Pandya DJ (2012) Pharmacognostic and phytochemical study of aerial parts of Carissa carandas. Int J Biol Pharm Res 3:75–81Google Scholar
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  20. Naim Z, Khan MA, Nizami SS (1988) Isolation of a new isomer of ursolic acid from fruits and leaves of Carissa carandas. Pak J Sci Ind Res 31:753–755Google Scholar
  21. Paladi RK, Rai AN, Penna S (2017) Silver nitrate modulates organogenesis in Brassica juncea (L.) through differential antioxidant defense and hormonal gene expression. Sci Hortic 226:61–267CrossRefGoogle Scholar
  22. Patil RP, Pai SR, Pawar NV, Shimpale VB, Patil RM, Nimbalkar MS (2012) Chemical characterization, mineral analysis, and antioxidant potential of two underutilized berries (Carissa carandus and Eleagnus conferta) from the Western Ghats of India. Crit Rev Food Sc Nutr 52:312–320CrossRefGoogle Scholar
  23. Purohit SD, Kukda G (2004) Micropropagation of an adult tree—Wrightia tinctoria. Indian J Biotechnol 3:216–220Google Scholar
  24. Rai R, Misra KK (2005) Micropropagation of Karonda (Carissa carandas) through shoot multiplication. Sci Hortic 103:227–232CrossRefGoogle Scholar
  25. Raut UA, Jadhav GG, Bhogave AF, Deshmukh MS (2015) Effect of different IBA levels on air layering of karonda (Carissa carandas L.). Res Crops 16:537–541CrossRefGoogle Scholar
  26. Singh SK, Syamal MM (2000) Anti-auxin enhance Rosa hybrida L. micropropagation. Biol Plant 43:279–281CrossRefGoogle Scholar
  27. Vanajalatha K (2013) Studies on the propagation of karonda (Carissa carandas L.) (Doctoral dissertation, Dr. YSR Horticultural University). http://krishikosh.egranth.ac.in/handle/1/69602d-

Copyright information

© The Society for In Vitro Biology 2018

Authors and Affiliations

  • Bhushan S. Bhadane
    • 1
  • Vijay L. Maheshwari
    • 2
  • Ravindra H. Patil
    • 1
    Email author
  1. 1.Plant Tissue Culture Laboratory, Department of Microbiology and BiotechnologyR. C. Patel Arts, Commerce and Science CollegeShirpurIndia
  2. 2.Department of Biochemistry, School of Life SciencesNorth Maharashtra UniversityJalgaonIndia

Personalised recommendations