Micropropagation of Glossonema varians (Stocks) Benth. ex Hook.f.—a rare Asclepiadeae of Indian Thar Desert
In the present study, an in vitro regeneration protocol for Glossonema varians (Stocks) Benth. ex Hook.f. of family Asclepiadaceae was optimized. Cotyledonary nodes of in vitro cultured seedlings were used as explants for activation of axillary shoot buds. Axillary shoot buds were initially activated on 0.1 mg L−1 6-benzylaminopurine (BAP) and then multiplied on 0.05 mg L−1 BAP. Shoots were rooted in vitro on 1/4 strength Murashige and Skoog medium containing 0.1 mg L−1 2-naphthoxyacetic acid and 100 mg L−1 activated charcoal. The cultures were maintained in a 12 h photoperiod at 40–50 μmol m−2 s−1 spectral flux photon, 25–30 ± 2°C, and 60% relative humidity (RH). Up to 80% of in vitro regenerated plantlets were acclimatized on soilrite in cotton-plugged culture tubes in the greenhouse. This protocol can be a useful method to mass propagate and conserve this rare plant to balance biodiversity in the desert ecosystem.
KeywordsGlossonema varians Rare plant Dodha Cotyledonary node In vitro regeneration
The authors acknowledge the Department of Biotechnology (DBT), Government of India, for establishment of regional facilities for Micropropagation and Hardening of plants of arid regions in the Department of Botany, Jai Narain Vyas University, Jodhpur, India.
- Al Hadidi SH, Khan RS, Qaradawi A, Alsafran MH, Ahmad TA (2017) Efficient plantlet regeneration of Glossonema edule, a critically endangered desert plant. Glob J Agric Res Rev 5:269–277Google Scholar
- Dagla HR, Shekhawat NS (2005a) Plant regeneration from tissue culture of Chloris virgata: a salt-tolerant desert grass. Indian J Biotechnol 4:400–403Google Scholar
- Dagla HR, Shekhawat NS (2005b) In vitro multiplication of Haloxylon recurvum (Moq.)—a plant for saline soil reclamation. J Plant Biotechnol 7:1–5Google Scholar
- Dagla HR, Paliwal A, Shekhawat NS (2007) Oran: a sacred way for biodiversity conservation in Indian Thar Desert. Curr Sci 93:279–280Google Scholar
- Dagla HR, Nair S, Vyas DK, Upendra JM (2014) In vitro culture of plants from arid environments. In: Tuteja N, Gill SS (eds) Climate change and abiotic stress tolerance. Wiley-VCH Verlag, Germany, pp 933–938Google Scholar
- Gehlot HS, Tak N, Dagla HR, Davis TD (2014) Indigenous and modern scientific strategies for characterization, conservation and sustainable utilization of bio-resources of the Indian Thar Desert. J Arid Land Stud 24:5–8Google Scholar
- Jukanti A K, Dagla HR, Kalwani P, Goswami D, Upendra J M, Kalia RK, Bhatt R K (2017) Grain protein estimation and SDS-PAGE profiling of six important arid legumes. Legume Res- Int J 40:485–490Google Scholar
- Nair S, Dagla HR (2016) Cloning of desert shrub Leptadenia pyrotechnica from mature shoots and their genetic stability analyses by RAPD, ISSR and ISJ markers. Indian J Biotechnol 15:427–432Google Scholar
- Upendra JM, Rao SR, Dagla HR (2017) Genetic diversity analysis of Salvadora persica: an evergreen halo-xeric species of semi-arid and sub-humid regions of Rajasthan, India. Ecol Genet Genom 2:35–41Google Scholar