In Vitro Cellular & Developmental Biology - Plant

, Volume 54, Issue 6, pp 637–641 | Cite as

Micropropagation of Glossonema varians (Stocks) Benth. ex Hook.f.—a rare Asclepiadeae of Indian Thar Desert

  • Aarti Paliwal
  • Narpat S. Shekhawat
  • Harchand R. DaglaEmail author
Plant Tissue Culture


In the present study, an in vitro regeneration protocol for Glossonema varians (Stocks) Benth. ex Hook.f. of family Asclepiadaceae was optimized. Cotyledonary nodes of in vitro cultured seedlings were used as explants for activation of axillary shoot buds. Axillary shoot buds were initially activated on 0.1 mg L−1 6-benzylaminopurine (BAP) and then multiplied on 0.05 mg L−1 BAP. Shoots were rooted in vitro on 1/4 strength Murashige and Skoog medium containing 0.1 mg L−1 2-naphthoxyacetic acid and 100 mg L−1 activated charcoal. The cultures were maintained in a 12 h photoperiod at 40–50 μmol m−2 s−1 spectral flux photon, 25–30 ± 2°C, and 60% relative humidity (RH). Up to 80% of in vitro regenerated plantlets were acclimatized on soilrite in cotton-plugged culture tubes in the greenhouse. This protocol can be a useful method to mass propagate and conserve this rare plant to balance biodiversity in the desert ecosystem.


Glossonema varians Rare plant Dodha Cotyledonary node In vitro regeneration 



The authors acknowledge the Department of Biotechnology (DBT), Government of India, for establishment of regional facilities for Micropropagation and Hardening of plants of arid regions in the Department of Botany, Jai Narain Vyas University, Jodhpur, India.


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Copyright information

© The Society for In Vitro Biology 2018

Authors and Affiliations

  • Aarti Paliwal
    • 1
  • Narpat S. Shekhawat
    • 1
  • Harchand R. Dagla
    • 1
    Email author
  1. 1.Plant Biotechnology and Molecular Biology Laboratory, Biotechnology Unit Department of BotanyJai Narain Vyas UniversityJodhpurIndia

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