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In Vitro Cellular & Developmental Biology - Plant

, Volume 54, Issue 6, pp 621–625 | Cite as

Efficient callus-mediated regeneration and in vitro root tuberization in Trichosanthes kirilowii Maxim., a medicinal plant

  • Fenglan Zhao
  • Rong Wang
  • Jianping Xue
  • Yongbo DuanEmail author
Plant Tissue Culture
  • 190 Downloads

Abstract

Trichosanthes kirilowii Maxim. is a climbing herb with considerable medicinal value. In this study, efficient protocols for callus-mediated regeneration and in vitro tuberization of this plant were developed. Sterilized stem and leaf tissues were cultured on Murashige and Skoog (MS) medium with plant growth regulators (PGRs), and additives that promoted callus induction and regeneration. Both stem and leaf tissues showed the best response (100%) for callus initiation on MS medium supplemented with 4.5-μM 2,4-dichlorophenoxyacetic acid (2,4-D). Efficient shoot organogenesis was obtained by exposing the callus tissue to 4.6-μM kinetin, 2.2-μM 6-benzylaminopurine, and 2.7-μM 1-naphthylacetic acid (NAA) along with 12.6-μM copper sulfate, which yielded a shoot regeneration rate of 85.5% and 28 shoots derived from each callus. In vitro shoots were best rooted on half-strength (1/2) MS medium with 2.7-μM NAA. Tuberous roots were efficiently induced on rooting medium with 5% (w/v) sucrose under short illumination conditions (8 h photoperiod). Rooted plantlets were successfully acclimatized in pots with a > 90% survival rate. This protocol provides an effective method for callus-mediated regeneration and in vitro root tuberization.

Keywords

Trichosanthes kirilowii Callus Shoot organogenesis Copper sulfate In vitro root tuberization 

Notes

Funding information

This work was supported by the National Natural Science Foundation of China (31501368, 81573518), the Anhui Provincial Natural Science Foundation, China (1608085MC52), and the Project of Natural Science Research of Universities in Anhui Province, China (KJ2016B016).

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Copyright information

© The Society for In Vitro Biology 2018

Authors and Affiliations

  • Fenglan Zhao
    • 1
  • Rong Wang
    • 1
  • Jianping Xue
    • 1
  • Yongbo Duan
    • 1
    Email author
  1. 1.Key Laboratory of Resource Plant Biology of Anhui Province, College of Life SciencesHuaibei Normal UniversityHuaibeiChina

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