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Overexpression of cinnamyl alcohol dehydrogenase gene from sweetpotato enhances oxidative stress tolerance in transgenic Arabidopsis

  • Young-Hwa Kim
  • Gyung-Hye Huh
Genetic Transformation
  • 8 Downloads

Abstract

Cinnamyl alcohol dehydrogenase (CAD) is the enzyme in the last step of lignin biosynthetic pathway and is involved in the generation of lignin monomers. IbCAD1 gene in sweetpotato (Ipomoea batatas) was identified, and its expression was induced by abiotic stresses based on promoter analysis. In this study, transgenic Arabidopsis plants overexpressing IbCAD1 directed by CaMV 35S promoter were developed to determine the physiological function of IbCAD1. IbCAD1-overexpressing transgenic plants exhibited better plant growth and higher biomass compared to wild type (WT), under normal growth conditions. CAD activity was increased in leaves and roots of transgenic plants. Sinapyl alcohol dehydrogenase activity was induced to a high level in roots, which suggests that IbCAD1 may regulate biosynthesis of syringyl-type (S) lignin. Lignin content was increased in stems and roots of transgenic plants; this increase was in S lignin rather than guaiacyl (G) lignin. Overexpression of IbCAD1 in Arabidopsis resulted in enhanced seed germination rates and tolerance to reactive oxygen species (ROS), such as hydrogen peroxide (H2O2). Taken together, our results show that IbCAD1 controls lignin content by biosynthesizing S units and plays an important role in plant responses to oxidative stress.

Keywords

Cinnamyl alcohol dehydrogenase Lignin ROS Syringyl Sweetpotato 

Notes

Funding information

This work was supported by the 2015 Inje University research grant.

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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Ubiquitous healthcare research centerInje UniversityGimhaeRepublic of Korea
  2. 2.Institute of Digital Anti-Aging HealthcareGraduate School of Inje UniversityGimhaeRepublic of Korea
  3. 3.Department of Healthcare Information TechnologyInje UniversityGimhaeRepublic of Korea

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