Advertisement

Plant growth regulators improve in vitro flowering and rapid generation advancement in lentil and faba bean

  • Saeid Hassanzadeh Mobini
  • Monika Lulsdorf
  • Thomas D. Warkentin
  • Albert VandenbergEmail author
Plant Development/Regeneration

Abstract

Rapid generation technology (RGT) involves applying plant growth regulators to accelerate in vitro flowering and the use of immature seed to shorten the time required to produce the next generation of plants. The effect of different concentrations of flurprimidol and combinations of one cytokinin and two auxins on in vitro flowering was evaluated using two lentil (Lens culinaris Medik.) and two faba bean (Vicia faba L.) cultivars. Adding flurprimidol to the medium reduced the internode length of plants, and plant height was decreased to approximately 10 cm in both species. The optimal concentration of flurprimidol depended on the species and the light intensity. The combination of 0.3 μM flurprimidol, 5.7 μM indole-3-acetic acid, and 2.3 μM zeatin resulted in 100% of faba bean plants flowering and 90% setting seed. The combination of 0.9 μM flurprimidol, 0.05 μM 4-chloroindole-3-acetic acid, and with a perlite growth substrate resulted in 90% of lentil plants flowering and over 80% with seed set. However, faba bean showed better response with agar as the substrate. Under optimized conditions, a single generation cycle was achieved in 54 d for faba bean and 45 d for lentil. RGT could produce seven and eight generations per year for faba bean and lentil, respectively. For the single seed descent breeding method for self-pollinated plants, a seed of each plant in each generation is advanced three times per year until near-homozygosity, thus requiring more than 2 yr. The RGT method produces about double the number of generations per year and therefore has potential for significant acceleration of pulse crop breeding programs.

Keywords

4-Chloroindole-3-acetic acid Faba bean Flowering Flurprimidol Indole-3-acetic acid Lentil Rapid regeneration Zeatin 

Notes

Acknowledgments

The financial support of the Saskatchewan Pulse Growers and the Alberta Pulse Growers Commission, the cooperation of the Crop Development Centre, University of Saskatchewan, and the technical expertise of Shermy B. Mudiyanselage are gratefully acknowledged.

References

  1. Ali B, Hayat S, Aiman Hasan S, Ahmad A (2008) A comparative effect of IAA and 4-Cl-IAA on growth, nodulation and nitrogen fixation in Vigna radiata (L.) Wilczek. Acta Physiol Plant 30:35–41CrossRefGoogle Scholar
  2. Bassil NV, Mok D, Mok MC (1993) Partial purification of a cis-trans-isomerase of zeatin from immature seed of Phaseolus vulgaris L. Plant Physiol 102:867–872PubMedCentralPubMedGoogle Scholar
  3. Croser J, Lulsdorf M, Grewal R, Usher K, Siddique KM (2011) Isolated microspore culture of chickpea (Cicer arietinum L.): induction of androgenesis and cytological analysis of early haploid divisions. In Vitro Cell Dev Biol-Plant 47:357–368CrossRefGoogle Scholar
  4. El-Saeid H, Abouziena HF, AbdAlla M (2011) Effect of some bioregulators on white lupine (Lupinus termis) seed yield and its components and on endogenous hormones content in seeds. Electron J Pol Agric Univ 14:2Google Scholar
  5. Emery RJN, Leport L, Barton JE, Turner NC, Atkins CA (1998) cis-Isomers of cytokinin predominate in chickpea seeds throughout their development. Plant Physiol Biochem 117:1515–1523Google Scholar
  6. Faisal M, Siddique I, Anis M (2006) In vitro rapid regeneration of plantlets from nodal explants of Mucuna pruriens—a valuable medicinal plant. Ann Appl Biol 148:1–6CrossRefGoogle Scholar
  7. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158CrossRefPubMedGoogle Scholar
  8. Khalil S, El-Saeid H, Shalaby M (2006) The role of kinetin in flower abscission and yield of lentil plant. J Appl Sci Res 2:587–591Google Scholar
  9. Levene H (1960) Robust tests for equality of variances. In: Olkin I, Ghurye SG, Hoeffding W, Madow WG, Mann HB (eds) Contributions to probability and statistics: essays in honor of Harold Hotelling. Stanford University Press, Redwood City, CA, pp 278–292Google Scholar
  10. Mamidala P, Nanna RS (2009) Efficient in vitro plant regeneration, flowering and fruiting of dwarf tomato cv. Micro-Msk. Plant Omics 2:98–102Google Scholar
  11. McDonald JH (2009) Handbook of biological statistics, vol 2. Sparky House, Baltimore, MDGoogle Scholar
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  13. Ochatt S, Sangwan R (2008) In vitro shortening of generation time in Arabidopsis thaliana. Plant Cell Tissue Organ Cult 93:133–137CrossRefGoogle Scholar
  14. Ochatt S, Sangwan RS, Marget P, Ndong YA, Rancillac M, Perney P, Röbbelen G (2002) New approaches towards the shortening of generation cycles for faster breeding of protein legumes. Plant Breed 121:436–440CrossRefGoogle Scholar
  15. Quesnelle PE, Emery RJN (2007) cis-Cytokinins that predominate in Pisum sativum during early embryogenesis will accelerate embryo growth in vitro. Can J Bot 85:91–103CrossRefGoogle Scholar
  16. Ribalta FM, Croser JS, Erskine W, Finnegan PM, Lulsdorf MM, Ochatt SJ (2014) Antigibberellin-induced reduction of internode length favors in vitro flowering and seed-set in different pea genotypes. Biol Plant 58:39–46CrossRefGoogle Scholar
  17. Samoylov VM, Tucker DM, Thibaud-Nissen F, Parrott WA (1998) A liquid-medium-based protocol for rapid regeneration from embryogenic soybean cultures. Plant Cell Rep 18:49–54CrossRefGoogle Scholar
  18. Sinclair A (2011) SAS Institute Inc v world programming limited. European law reports 15:303–314CrossRefGoogle Scholar
  19. Slater SM, Yuan HY, Lulsdorf MM, Vandenberg A, Zaharia LI, Han X, Abrams SR (2013) Comprehensive hormone profiling of the developing seeds of four grain legumes. Plant Cell Rep 32:1939–1952CrossRefPubMedGoogle Scholar
  20. Yonekura-Sakakibara K, Kojima M, Yamaya T, Sakakibara H (2004) Molecular characterization of cytokinin-responsive histidine kinases in maize. Differential ligand preferences and response to cis-zeatin. Plant Physiol 134:1654–1661CrossRefPubMedCentralPubMedGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2014

Authors and Affiliations

  • Saeid Hassanzadeh Mobini
    • 1
  • Monika Lulsdorf
    • 1
  • Thomas D. Warkentin
    • 1
  • Albert Vandenberg
    • 1
    Email author
  1. 1.Crop Development CentreUniversity of SaskatchewanSaskatoonCanada

Personalised recommendations