Impact of carbon and phosphate starvation on growth and programmed cell death of maritime pine suspension cells

  • Herlânder Azevedo
  • Pedro Humberto Castro
  • Joana Ferreira Gonçalves
  • Teresa Lino-Neto
  • Rui Manuel Tavares
Physiology

Abstract

Programmed cell death is a fundamental aspect of plant development and defense. In suspension cultures of maritime pine (Pinus pinaster Ait.), cell death was associated with the simultaneous depletion of sugar and phosphate. This present work suggests that sugar rather than phosphate deprivation induced programmed cell death events, including degradation of nuclear DNA and remobilization of phosphate. However, phosphate starvation may have a synergistic effect on programmed cell death mediated by the lack of carbon source. Sugar and phosphate analogs were used to evaluate the nature of signaling events, and results suggested that programmed cell death induction by sugar starvation occurs downstream of hexokinase-based sugar sensing mechanisms, and that the synergistic effect of lack of phosphate is independent of phosphate sensing.

Keywords

Pinus pinaster Programmed cell death PCD Phosphate Sugar 

Notes

Acknowledgments

H. A. was supported by the project “Genomics and Evolutionary Biology” co-financed by North Portugal Regional Operational Programme 2007/2013 (ON.2–O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF). P.H.C. was supported by Fundação para a Ciência e Tecnologia, grant reference SFRH/BD/44484/2008.

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Copyright information

© The Society for In Vitro Biology 2014

Authors and Affiliations

  • Herlânder Azevedo
    • 1
  • Pedro Humberto Castro
    • 2
  • Joana Ferreira Gonçalves
    • 2
  • Teresa Lino-Neto
    • 2
  • Rui Manuel Tavares
    • 2
  1. 1.CIBIO, Centro de Investigação em Biodiversidade e Recursos GenéticosUniversidade do PortoVairãoPortugal
  2. 2.Center for Biodiversity, Functional & Integrative Genomics (BioFIG), Plant Functional Biology CenterUniversity of MinhoBragaPortugal

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