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In Vitro Cellular & Developmental Biology - Plant

, Volume 49, Issue 6, pp 759–764 | Cite as

Suppression of ethylene levels promotes morphogenesis in pepper (Capsicum annuum L.)

  • Diego Silva Batista
  • Leonardo Lucas Carnevalli Dias
  • Amanda Ferreira Macedo
  • Mailson Monteiro do Rêgo
  • Elizanilda Ramalho do Rêgo
  • Eny Iochevet Segal Floh
  • Fernando Luiz Finger
  • Wagner Campos Otoni
Physiology

Abstract

Ethylene and polyamines (PAs) are two phytohormones that play important roles during in vitro morphogenesis of several plant species. The interaction between ethylene and PAs has been of interest because both have S-adenosylmethionine as a precursor. To study the influence of ethylene and PAs on in vitro morphogenesis of an ornamental pepper, we added an ethylene scavenger, PAs, a PA inhibitor, and compounds that affect ethylene biosynthesis and activity to the regeneration medium. Regeneration frequencies increased in response to treatment with ethylene inhibitors (aminoethoxyvinylglycine and silver thiosulfate) and an ethylene scavenger (mercury perchlorate). Treatment with the ethylene precursor 1-aminocyclopropane-1-carboxylic acid reduced the regeneration frequency, increased callus formation, and increased ethylene levels; similar results were obtained in response to treatment with the PA inhibitor methylglyoxal-bis(guanylhydrazone). By contrast, treatment with PAs (particularly spermidine and spermine) decreased ethylene levels, increased the regeneration frequency, and increased shoot bud formation. These results suggest a coordinated regulation of ethylene and polyamines because the suppression of ethylene levels using ethylene inhibitors, polyamines, or mercury perchlorate increased the in vitro regeneration frequency and morphogenic responses of Capsicum annuum L.

Keywords

Morphogenesis Plant regeneration Putrescine Spermidine Spermine Phytohormone crosstalk 

Notes

Acknowledgments

The authors thank the Brazilian sponsoring agencies CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil), FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior) for financial support.

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Copyright information

© The Society for In Vitro Biology 2013

Authors and Affiliations

  • Diego Silva Batista
    • 1
  • Leonardo Lucas Carnevalli Dias
    • 1
  • Amanda Ferreira Macedo
    • 2
  • Mailson Monteiro do Rêgo
    • 3
  • Elizanilda Ramalho do Rêgo
    • 3
  • Eny Iochevet Segal Floh
    • 2
  • Fernando Luiz Finger
    • 4
  • Wagner Campos Otoni
    • 1
  1. 1.Laboratório de Cultura de Tecidos Vegetais (LCTII), Departamento de Biologia Vegetal/BIOAGROUniversidade Federal de ViçosaViçosaBrazil
  2. 2.Laboratório de Biologia Celular (BIOCEL), Instituto de Ciências Biológicas, Departamento de BotânicaUniversidade de São Paulo (USP)São PauloBrazil
  3. 3.Centro de Ciências AgráriasUniversidade Federal da ParaíbaAreiaBrazil
  4. 4.Departamento de FitotecniaUniversidade Federal de ViçosaViçosaBrazil

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