In Vitro Cellular & Developmental Biology - Plant

, Volume 48, Issue 6, pp 620–626 | Cite as

Micropropagation of Mandevilla moricandiana (A.DC.) Woodson

  • Sandra Zorat Cordeiro
  • Naomi Kato Simas
  • Anaize Borges Henriques
  • Celso Luiz Salgueiro Lage
  • Alice Sato
Protocols/Methods
First Online:
Received:
Accepted:

Abstract

A protocol was developed for micropropagation of Mandevilla moricandiana (A.DC.) Woodson, a native plant from Brazil. Shoots, obtained from in vitro plantlets were used as source of nodal segments for shoot production from axillary buds. The nodal segments were grown on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine and/or indole-3-acetic acid to induce axillary bud elongation. After a 2-mo culture period, the medium supplemented with 1.0 mg L−1 6-benzyladenine gave the largest number of nodal segments per explant. The nodal segments obtained from plants developed under these conditions were grown on medium supplemented with different concentrations indole-3-acetic acid, α-naphthaleneacetic acid, and indole-3-butyric acid. The use of the medium supplemented with indole-3-acetic acid and indole-3-buryric induced shoot elongation and shoot development, formation of basal callus, and/or indirect organogenesis of roots. Following transfer of shoots to soil, the plants with only basal callus showed 10% survival and developed roots from callus, while in vitro-rooted plants had a maximum 40% survival rate ex vitro. Regardless of the auxin added to the rooting medium, the acclimatization period allowed the plants rooted in vitro to develop their shoots fully. The protocol developed here is suitable for the production of shoots and rooted plantlets of M. moricandiana.

Keywords

Mandevilla Apocynaceae Micropropagation Morphogenesis Tissue culture 
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Notes

Acknowledgments

The authors thank the Conselho de Administração de Pessoal de Ensino Superior for a doctoral scholarship for the first author, Programa de Pós-graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro for financial support, Dr. Tatiana Ungaretti Paleo Konno of the UFRJ-Macaé for providing seeds of M. moricandiana, taxonomists Dr. Jorge Fontella Pereira of the Museu Nacional (UFRJ), Marcelo Fraga Castilhiori and Inaldo do Espírito Santo of the Herbarium Bradeanum for species identification, Universidade Federal do Estado do Rio de Janeiro (UNIRIO) for providing transport to the collection areas, IBAMA-Brazilian Institute for Environment and Natural Renewable Resources-for authorization to collect (Scientific Research Activities no. 18498-1), and the anonymous reviewers for their valuable comments and suggestions to improve the manuscript.

References

  1. Adams M, Gmünder F, Hamburger M (2007) Plants traditionally used in age related brain disorders-a survey of ethnobotanical literature. J Ethnopharmacol 113:363–381Google Scholar
  2. Alves M, Oliveira AS (1992) Contribuição ao estudo dos aspectos morfológicos de Mandevilla fragrans (Stadelm.) Woodson. Atas Soc Bot Bras 3:101–106Google Scholar
  3. Amaral CLF, Silva AB (2003) Melhoramento Biotecnológico de Plantas Medicinais. Biotecnologia Cienc Desenvolv 30:55–59Google Scholar
  4. Appezzato-da-Glória B, Estelita MME (2000) The developmental anatomy of the subterranean system in Mandevilla illustris (Vell.) Woodson and M. velutina (Mart. Ex Stadelm) Woodson (Apocynaceae). Braz J Bot 23:27–35Google Scholar
  5. Biondo R, Soares AM, Bertoni BW, França SC, Pereira MAS (2004) Direct organogenesis of Mandevilla illustris (Vell.) Woodson and effects of its aqueous extract on the enzymatic and toxic activities Crotalus durissus terrificus snake venom. Plant Cell Rep 22:549–552PubMedCrossRefGoogle Scholar
  6. Biondo R, Souza AV, Bertoni BW, Soares AM, França SC, Pereira MAS (2007) Micropropagation, seed propagation and germplasm bank of Mandevilla velutina (Mart.) Woodson. Sci Agric (Piracicaba, Braz) 64:263–268Google Scholar
  7. Boutebtoub W, Chevalier M, Mauget JC, Signone M, Morel P, Galopin G (2009) Localizing starch reserves in Mandevilla sanderi (Hemsl.) Woodson using a combined histochemical and biochemical approach. HortSci 44:1879–1883Google Scholar
  8. Calixto JB, Nicolau M, Yunes RA (1985) The selective antagonism of bradykinin action on rat isolated uterus by crude Mandevilla velutina extract. Br J Pharmacol 85:729–731PubMedCrossRefGoogle Scholar
  9. Handro W, Floh EIS, Ferreira CM, Guerra MP (1988) Tissue, cell culture and micropropagation of Mandevilla velutina, a natural source of a bradykinin antagonist. Plant Cell Rep 7:564–566CrossRefGoogle Scholar
  10. Martins S, Alves M (2008) Aspectos anatômicos de espécies simpátridas de Mandevilla (Apocynaceae) ocorrentes em inselbergues de Pernambuco-Brasil. Rodriguésia 59:369–380Google Scholar
  11. Medeiros JD (2003) A biotecnologia e a extinção das espécies. Biotecnologia Cienc Desenvolv 30:109–113Google Scholar
  12. Metcalfe CR, Chalke L (1950) Anatomy of the dicotyledons: leaves, stem and wood in relation to taxonomy with notes on economic uses. Claredon, OxfordGoogle Scholar
  13. Murashige T, Skoog FA (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  14. Nishitha IK, Martin KP, Ligimol L, Shahanaz-Beegum A, Madhusoodanan PV (2006) Micropropagation and encapsulation of medicinally important Chonemorpha grandiflora. In Vitro Cell Dev Biol-Plant 42:385–388CrossRefGoogle Scholar
  15. Pereira-Netto AB (1996) In vitro propagation of Hancornia speciosa, a tropical fruit-tree. In Vitro Cell Dev Biol-Plant 32:253–256CrossRefGoogle Scholar
  16. Pioker F, Taniguchi M, Prato M, Kerpel S (2010) Biologia floral e síndrome de polinização de Mandevilla moricandiana (A.DC.) Woodson. In: Viana BF, Silva FO (eds) Biologia e Ecologia da Polinização: Cursos de campo, vol 2. Rede Bahiana de Polinizadores/EDUFBA, Salvador, pp 193–204Google Scholar
  17. Raha S, Roy SC (2001) In vitro plant regeneration in Holarrhena antidysenterica Wall., through high-frequency axillary shoot proliferation. In Vitro Cell Dev Biol-Plant 37:232–236CrossRefGoogle Scholar
  18. Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153PubMedCrossRefGoogle Scholar
  19. Rout GR, Samantaray S, Das P (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol Adv 18:91–120PubMedCrossRefGoogle Scholar
  20. Sales MF, Kinoshita LS, Simões AO (2006) Eight new species of Mandevilla Lindley (Apocynaceae, Apocynoideae) from Brazil. Novon 16:112–128CrossRefGoogle Scholar
  21. Santos MG, Fevereiro PCA, Reis GL, Barcelos JI (2009) Recursos vegetais da Restinga de Carapebus, Rio de Janeiro, Brasil. Biota Neotrop 6:35–54Google Scholar
  22. Sudha CG, Krishnan PN, Pushpangadan P, Seeni S (2005) In vitro propagation of Decalepis arayalpathra, a critically endangered ethnomedicinal plant. In Vitro Cell Dev Biol-Plant 41:648–654CrossRefGoogle Scholar
  23. Teixeira JB, Cruz ARR, Ferreira FR, Cabral JRS (2001) Biotecnologia aplicada à produção de mudas. Biotecnol Ciênc Desenvolv 19:42–47Google Scholar
  24. Wiersema JH, León B (1999) World economic plants: a standard reference. CRC, Boca RatonGoogle Scholar
  25. Woodson RE Jr (1933) Studies in the Apocynaceae. IV. The American Genera of the Echitoideae. Ann Mo Bot Gard 20:605–790CrossRefGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2012

Authors and Affiliations

  • Sandra Zorat Cordeiro
    • 1
  • Naomi Kato Simas
    • 2
  • Anaize Borges Henriques
    • 3
  • Celso Luiz Salgueiro Lage
    • 4
  • Alice Sato
    • 5
  1. 1.Post-graduation in Plant BiotechnologyFederal University of Rio de JaneiroRio de JaneiroBrazil
  2. 2.Department of Natural Products and Food–College of PharmacyFederal University of Rio de JaneiroRio de JaneiroBrazil
  3. 3.Laboratory of Plant Development Physiology–Institute of BiologyFederal University of Rio de JaneiroRio de JaneiroBrazil
  4. 4.National Institute of Industrial PropertyRio de JaneiroBrazil
  5. 5.Laboratory of Plant Tissue Culture–Department of BotanyFederal University of the State of Rio de JaneiroRio de JaneiroBrazil

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