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A two-step procedure for adventitious shoot regeneration on excised leaves of lowbush blueberry

  • S. C. DebnathEmail author
Article

Abstract

An efficient system to regenerate shoots on excised leaves of greenhouse-grown wild lowbush blueberry (Vaccinium angustifolium Ait.) was developed in vitro. The effect of thidiazuron (TDZ) on adventitious bud and shoot formation from apical, medial, and basal segments of the leaves was tested. Leaf cultures produced multiple buds and shoots with or without an intermediary callus phase on 2.3–4.5 μM TDZ within 6 wk of culture initiation. The greatest shoot regeneration came from young expanding basal leaf segments positioned with the adaxial side touching the culture medium and maintained for 2 wk in darkness. Callus development and shoot regeneration depended not only on the polarity of the explants but also on the genotype of the clone that supplied the explant material. TDZ-initiated cultures were transferred to medium containing 2.3–4.6 μM zeatin and produced usable shoots after one additional subculture. Elongated shoots were dipped in 39.4 mM indole-3-butyric acid powder and planted on a peat:perlite soilless medium at a ratio of 3:2 (v/v), which yielded an 80–90% rooting efficiency. The plantlets were acclimatized and eventually established in the greenhouse with 75–85% survival.

Keywords

Organogenesis Vaccinium angustifolium Cytokinins In vitro culture Ex vitro rooting 

Notes

Acknowledgments

The author gratefully acknowledges the excellent technical help from Glen Chubbs, Sarah Leonard, Darryl Martin, Shawn Foley, and Jeff Power. This work is the Atlantic Cool Climate Crop Research Centre contribution no. 187.

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Copyright information

© The Society for In Vitro Biology 2009

Authors and Affiliations

  1. 1.Atlantic Cool Climate Crop Research CentreAgriculture and Agri-Food CanadaSt. John’sCanada

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