In vitro germination and micropropagation of Givotia rottleriformis Griff.
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The propagation of Givotia rottleriformis Griff. is difficult as a result of long seed dormancy associated with poor seed germination. The present study was undertaken to develop a protocol to overcome seed dormancy by culture of zygotic embryo axes and then develop an efficient method for micropropagation of Givotia. Best germination frequency (78.3%) was achieved from mature zygotic embryo axes isolated from acid-scarified fresh seeds when cultured on Murashige and Skoog (MS) medium (half-strength major salts) with 28.9 μM gibberellic acid (GA3). Efficient plant conversion was achieved by transfer of 10-d-old germinated embryos to MS medium (half-strength major salts) supplemented with 1.2 μM kinetin (KN) and 0.5 μM indole-3-butyric acid (IBA). However, acid scarification of 1-yr-old seeds decreased the germination frequency of zygotic embryo axes in comparison to those obtained from non-acid-scarified seeds which germinated (96.2%) and converted into plants (80.3%) on MS basal (half-strength major salts) medium. Multiple shoot bud induction was achieved by culture of shoot tips derived from in vitro germinated seedlings on MS medium with 0.5 μM thidiazuron for 4 wk, and the shoots elongated after transfer to a secondary medium with 1.2 μM KN. A maximum number of 7.8 shoots per explant with an average shoot length of 3.2 cm was achieved after two subcultures on this medium. The in vitro regenerated shoots rooted (41.5%) on half-strength MS medium with 0.5 μM IBA. The in vitro generated seedlings and micropropagated plants were established in soil with a survival frequency of 70% and 60%, respectively.
KeywordsEuphorbiaceae Zygotic embryo axes Plant conversion Shoot tip culture Micropropagation
GP gratefully acknowledges the financial assistance received from the Institute of Life Sciences, Hyderabad and University Grants Commission, New Delhi to carry out this work. We express our thanks to Dr. Vara Prasad, Regional Forest Research Centre, Rajahmundry for permitting us to collect the seeds for the study. We acknowledge UGC-SAP and DST-FIST programmes of School of Life Sciences, University of Hyderabad for providing infrastructural requirements.
- Babitha, M.; Rao, P. S.; Elusing, M.; Sreenivasulu, A. Effect of auxins on rooting of stem cuttings of Givotia rottleriformis Griff. via hydropit. Indian For. 128: 266–270; 2002.Google Scholar
- Chang, S. H.; Yang, J. C. Enhancement of plant formation from embryo cultures of Taxus mairei using suitable culture medium and PVP. Bot. Bull. Acad. Sin. 37: 35–40; 1996.Google Scholar
- Hu, C. V.; Zanettini, M. H. B. Embryo culture and embryo rescue for wide cross hybrids. In: Gamborg O. L.; Phillips G. C. (eds) Plant cell tissue organ culture. Fundamental Methods. Springer-Verlag, Berlin, pp 129–141; 1995.Google Scholar
- Joshi, N. K.; Sharma, S.; Shamet, G. S.; Dhiman, R. C. Studies on the effect of auxins and season on rooting of stem cuttings of some important shrubs in nursery beds. Indian For. 118: 893–899; 1992.Google Scholar
- Kiran, D. J. S.; Rao, P. S.; Venkaiah, K.; Saibabu, A. Effect of GA3 on germination of Givotia rottleriformis Griff. seeds. Indian For. 127: 944–946; 2001.Google Scholar
- Kumar, A.; Sood, A.; Palni, U. T.; Gupta, A. K.; Palni, L. M. S. Micropropagation of Rosa domascena Mill from mature bushes using thidiazuron. J. Hortic. Sci. Biotechnol. 76: 30–34; 2001.Google Scholar
- Naidu, C. V.; Prasada Reddy, B. V.; Rao, P. S. Mineral composition of various components of seed of Givotia rottleriformis Griff. J. of Tropical Forestry. 17: 77–80; 2001.Google Scholar
- Nautiyal, S.; Singh, U.; Gurumurti, K. Rooting response of branch cuttings of teak (Tectona grandis) as influenced by season and growth hormone. Indian For. 117: 249–255; 1991.Google Scholar
- Nautiyal, S.; Singh, U.; Gurumurti, K. Rooting response of branch cuttings of teak (Tectona grandis) as influenced by growth hormones and position of cutting on the crown. Indian For. 118: 112–121; 1992.Google Scholar
- Nayar, M. P.; Ramamurthy, K.; Agarwal, V. S. Economic plants of India. Vol. 1 & II, Kolkata, India; 1989.Google Scholar
- Preece, J. E.; Imel, M. R. Plant regeneration from leaf explants of ‘Rhododendron’ P. J. M.. Hybrids. Sci. Hort. 48: 159–170; 1991.Google Scholar
- Rambabu, M.; Ujjwala, D.; Ugandhar, T.; Praveen, M.; Upender, M.; Ramaswamy, N. Effect of GA3 on enhancement of in vivo seed germination in Givotia rottleriformis (Euphorbiaceae)—an endangered forest tree. Indian For. 131: 25–30; 2005.Google Scholar
- Rao, P. S.; Bhanja, M.; Venkaiah, K. Propagation of toy making species of Andhra Pradesh. Booklet on Vision—2020. Research for Development, A. P. Forest Department; 1999.Google Scholar
- Reddy, R. A.; Mastan, M.; Naidu, C. V.; Rao, P. S. Seasonal variation in rooting response of stem cuttings of Givotia rottleriformis Griff. Ind. J. Forestry. 24: 475–477; 2001.Google Scholar
- Somasundaram, T. R. A handbook on the identification and description of trees, shrubs and some important herbs of the forests of the southern states. Southern Forest Rangers College, Coimbatore; 1980: 403 pp.Google Scholar
- Thammanna; Narayana Rao, K. Medicinal plants of Tirumala (first edition). Tirumala Tirupati Devasthanams (TTD), Tirupati, Andhra Pradesh; 1990.Google Scholar