Screening of a broad range of rice (Oryza sativa L.) germplasm for in vitro rapid plant regeneration and development of an early prediction system
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Rice has emerged as a model monocot for studies in agriculture and biotechnology due to its relatively small genome and a ready accessibility to plant material. Tissue culture is one of the tools required for genetic transformation and some breeding programs, and the selection of high-frequency regenerator types is essential for success in these technologies. Thirty-three rice entries with agricultural and biotechnological characteristics of interest were screened with the aim to identify the best regenerators. Entries that exhibited between 50% and 90% regeneration frequencies include ‘Taipei-309,’ ‘Super Dwarf,’ ‘Norin’ (japonica types), PI 312777, ‘Ali Combo’ (indica types), ‘STG-S,’ and ‘LA3’ (red rice types). One third of the entries tested were at least two times better at regeneration than the often-cited regenerator ‘Nipponbare.’ Those entries showing at least 85% frequency of greening or somatic embryo formation at 15 or 30 d on regeneration medium ultimately produced whole plants after 45 d on regeneration medium at high frequency (at least 40%); those entries not reaching the 85% threshold of greening by Days 15 or 30 exhibited moderate (15–40%) to low (less than 10%) frequency of whole plant regeneration. This greening response suggests the means for an early prediction system for identification of useful rice regenerator lines, which would be beneficial for high-throughput screening of germplasm as well as for decreasing the time and cost of in vitro culture.
KeywordsTissue culture Rice Early prediction Plant regeneration
The authors thank the USDA-ARS Dale Bumpers National Rice Research Center (Stuttgart, AR) for some rice varieties studied and partial financial support of this project; Dr. Karen Moldenhauer from the Arkansas Rice Research and Extension Center (University of Arkansas, Fayetteville, AR) for providing some rice varieties, Dr. James Oard (Louisiana State University, Baton Rouge, La) for providing a ‘Taipei-309’ source, and Dr. Katsutoshi Okuno from the Institute of Agrobiological Sciences (Ibaraki, Japan) for seeds of rice line ‘Daw-Dam.’ Research at the Phillips and Lorence groups is supported by grants to GP and AL from the Arkansas Biosciences Institute at Arkansas State University.
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