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Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl.

  • Peng Zhao
  • Fei Wu
  • Fo-Sheng Feng
  • Wan-Jun WangEmail author
Developmental/Biology/Morphogenesis

Abstract

An efficient system was established for a higher frequency of protocorm-like body (PLB) formation from the callus of Dendrobium candidum Wall ex Lindl. The calluses were induced from longitudinally bisected segments of protocorms and subcultured two times every 40d on Murashige and Skoog medium with macronutrients at half strength, micronutrients at full strength, 2% sucrose, and with 8.8μM 6-Benzylaminopurine. PLB formation was achieved when calluses were transferred onto the same basal medium without any plant growth regulators. PLBs developed into intact plantlets about 2cm in height and with four roots when on basal medium with 2.7μM 1-naphthaleneacetic acid. Plantlets were transplanted into vermiculite with a 95% survival rate in a greenhouse. Histological observations proved that globular somatic embryos could be produced from the inside and surface of the embryogenic callus during PLB formation.

Keywords

Differentiation Mass propagation Morphogenesis Orchid Somatic embryogenesis 

Notes

Acknowledgments

We thank Emily King for critical reading of the manuscript. This work was supported from the science foundation of Southwest Jiaotong University (X1200511130101).

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Copyright information

© The Society for In Vitro Biology 2007

Authors and Affiliations

  • Peng Zhao
    • 1
  • Fei Wu
    • 1
  • Fo-Sheng Feng
    • 1
  • Wan-Jun Wang
    • 1
    Email author
  1. 1.College of BioengineeringSouthwest Jiaotong UniversityChengduPeople’s Republic of China

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