Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume “Xue mei”

IAPB March/April 07
First Online:
Received:
Accepted:

Abstract

Using immature embryos and cotyledons as explants, a successful system to culture immature embryos and induce direct regeneration from cotyledons was established for Prunus mume “Xuemei”. For immature embryo culture, a high frequency of plantlet formation (89.5%) from the embryonic axis was obtained using half-strength Murashige and Skoog (1/2 MS) medium supplemented with 13.2 μM 6-benzyladenine (BA) and 2.7 μM 1-naphthaleneacetic (NAA). Shoots formed directly from cotyledons with the embryo axis intact when explants were cultured on 1/2 MS medium containing 2.2 μM BA with different combinations of NAA (2.7, 5.4 μM) and indole-3-butyric acid (IBA) (0, 2.5, 5.0 μM). Better results were achieved when the embryonic axis was removed from the cotyledons and cultured on 1/2 MS medium supplement with 13.2 μM BA, 2.7 μM NAA or 2.2 μM BA, 2.2 μM thidiazuron (TDZ), and 2.7 μM NAA, respectively. Regenerated shoots were successfully rooted on 1/2 MS or Woody Plant medium (WPM) supplemented with 2.5–5.0 μM IBA. The effect of the embryonic axis, BA, and TDZ on cotyledon regeneration was investigated in detail. Rooted plantlets were transferred to soil successfully.

Keywords

Cotyledons Direct shoot regeneration Immature embryos Prunus mume Organogenesis Rooting 
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Notes

Acknowledgments

We thank all the colleagues in our lab for constructive discussion and technical support, and are also grateful to all the staff of Wuhan P. mume garden, China for providing experimental materials.

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Copyright information

© The Society for In Vitro Biology 2007

Authors and Affiliations

  1. 1.Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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