In vitro propagation of limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot-tip, leaf- and inflorescence-node explants

  • C. L. Huang
  • M. T. Hsieh
  • W. C. Hsieh
  • A. P. Sagare
  • H. S. TsayEmail author


Rapid in vitro propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an endangered medicinal plant, was achieved by culturing the shoot-tip (primary and lateral), leaf- and influorescence-node explants. MS (Murashige and Skoog, 1962) medium supplemented with 8.87 μMN6-benyladenine (BA) and 1.07 μM α-naphthaleneacetic acid (NAA) supported induction of adventitious shoots from the shoot-tip, inflorescence-node and middle and basal parts of leaf explants after 60 d of culture. Adventitious shoots were multiplied by subculturing on MS medium supplemented with BA (2,21–17.75 μM) in combination with NAA (1.07 μM). The percentage of explants forming shoots and the average number of adventitious shoot buds produced per explant were stimulated by increasing the strength (1/4x, 1/2x, 1x, 2x) of the MS medium. Shoots were rooted on MS basal medium with 4.92 μM indole-3-butyric acid. Plantlets with a morphologically normal appearance produced from adventitious shoots were transferred to soil and acclimated in the growth chamber for 1 mo.

Key words

medicinal herb Limonium wrightii micropropagation Plumbaginaceae 


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  1. Agrawal, D. C.; Pawar, S. S.; Morwal, G. C.; Mascarenhas, A. F.. In vitro micropropagation of Delphinium malabaricum (Huth) Munz.-a rare species. Ann. Bot. 68:243–245 (1991).Google Scholar
  2. Bhuyan, A. K.; Pattnaik, S.; Chand, P. K.. Micropropagation of curry leaf tree [Murraya koenigii (L.) Spreng.] by axillary proliferation using intact seedlings. Plant Cell Rep. 16:779–782; 1997.CrossRefGoogle Scholar
  3. Erdei, I.; Kiss, Z.; Maliga, P. Rapid clonal multiplication of Digitalis lanata in tissue culture. Plant Cell Rep. 1:34–35; 1981.CrossRefGoogle Scholar
  4. Eay, M. F. Conservation of rarc and endangered plants using in vitro methods. In Vitro Cell. Dev. Biol. 28P:1–34; 1992.Google Scholar
  5. Hatano, K.; Shoyama, Y.; Nishioka, I. Multiplication of Pinellia ternata by callus culture of leaf segment. Shoyakugaku Zasshi 40:188–192; 1986.Google Scholar
  6. Hiraoka, N.; Kodama, T.; Tomita, Y.. In vitro propagation of Bupleurum falcatum. Shoyakugaku Zasshi 37:62–67; 1983.Google Scholar
  7. Hiraoka, N.; Oyanagi, M. In vitro propagation of Glehnia littoralis from shoot-tips. Plant Cell Rep. 7:39–42; 1988.CrossRefGoogle Scholar
  8. Hiraoka, N.; Yamada, N.; Kodama, T.; Tomita, Y. In vitro propagation of Atractylodes leancea. Plant Cell Rep. 3:85–87; 1984.CrossRefGoogle Scholar
  9. Huang, C.L., Investigation and study of the resources of Chinese medicinal materials grown in Taiwan (XVI): Investigation and study on the medicinal plants in Taitung county. MS dissertation, Taichung, Taiwan, Institute of Chinese Pharmaceutical Science, China Medical College, 1997.Google Scholar
  10. Kan, W. S. Manual of vegetale drugs in Taiwan, Part 2. Taipei, Taiwan: Medicine Publishing Inc.; 1978; 43–44.Google Scholar
  11. Kaul, K. Plant regeneration from cotyledon-hypocotyl explants of Pinus strobus L. Plant Cell Rep. 6:5–7; 1987.CrossRefGoogle Scholar
  12. Kitamura, Y.; Miura, H.; Sugii, M. Plant regeneration from callus cultures of Swertia pseudochinensis. Shoyakugaku Zasshi 43:256–258; 1989.Google Scholar
  13. Li, H. L. Plumbaginaceae. In: Li, H. L.; Liu, T. S.; Huang, T. C.; Koyama, T.; Devol, C. E. ed. Flora of Taiwan. Taipei, Taiwan: Epoch Publishing Co., Ltd.; 1978; 4:90–93.Google Scholar
  14. Lin, H. S.; De Jen, M. J.; Jacobsen, E. Direct shoot regeneration from excised leaf explants of in vitro grown seedlings of Alstroemeria L. Plant Cell Rep. 16:770–774; 1997.CrossRefGoogle Scholar
  15. Lu, C. Y.; Nugent, G.; Wardley, T. Efficient, direct plant regeneration from stem segments of chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple). Plant Cell Rep. 8:733–736; 1990.CrossRefGoogle Scholar
  16. Matsumoto, M.; Nagano, M.; Shoyama, Y.; Nishioka, I. New vegetative propagation method of Rehmannia glutinosa. Shoyakugaku Zasshi 40:193–197; 1986.Google Scholar
  17. Murashige, T.; Skoog, F. A revised medium for rapid and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.CrossRefGoogle Scholar
  18. Nishioka, I. Clonal multiplication of medicinal plants by tissue culture. Shoyakugaku Zasshi 42:1–11; 1988.Google Scholar
  19. Sunnichan, V. G.; Shivanna, K. R.; Mohan Ram, H. Y. Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis. Plant Cell Rep. 17:951–956; 1998.CrossRefGoogle Scholar
  20. Takayama, S.; Misawa, M. Differentiation in Lilium bulbscales grown in vitro. Effect of various cultural conditions. Physiol. Plant 46:184–190: 1979.CrossRefGoogle Scholar
  21. Tripepi, R. R. Adventitious shoot regeneration. In: Geneve, R. L.; Preece, J. E.; Merkle, S. A. ed. Biotechnology of ornamental plants. Wallingford, UK: CAB International; 1997; 45–71.Google Scholar
  22. Tsay, H. S.; Gau, T. G., Chen, C. C. Rapid clonal propagation of Pinellia ternata by tissue culture. Plant Cell Rep. 8:450–454; 1989.CrossRefGoogle Scholar
  23. Tsay, H. S.; Huang, H. L. Somatic embryo formation and germination from immature embryo-derived suspension-cultured cells of Angelica sinensis (Oliv.). Diels. Plant Cell Rep. 17:670–674; 1988.CrossRefGoogle Scholar
  24. van Altvorst, A. C.; Koehorst, H. J. J.; Bruinsma, T.; Dons, J. J. M. Improvement of adventitious shoot formation from carnation leaf explants. Plant Cell Tiss. Org. Cult. 37:87–90; 1994.CrossRefGoogle Scholar
  25. Wildi, E.; Schaffner, W.; Berger Büter, K. In vitro propagation of Petasites hybridus (Asteraceae) from leaf and petiole explants and inflorescence buds. Plant Cell Rep. 18:336–340; 1998.CrossRefGoogle Scholar

Copyright information

© Society for In Vitro Biology 2000

Authors and Affiliations

  • C. L. Huang
    • 1
  • M. T. Hsieh
    • 1
  • W. C. Hsieh
    • 1
  • A. P. Sagare
    • 2
  • H. S. Tsay
    • 2
    Email author
  1. 1.Institute of Chinese Pharmaceutical ScienceChina Medical CollegeTaichungTaiwan
  2. 2.Department of AgronomyTaiwan Agricultural Research InstituteTaichungTaiwan

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