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Primer mixture enhances PCR detection of Mycoplasma/acholeplasma contaminants in cell cultures

  • Jane Tang
  • Mengdong Hu
  • Sunmin Lee
  • Richard Roblin
Letter To The Editor Scientific Reports

Keywords

Mycoplasma Contamination Mycoplasma Infection Mycoplasma Species Prime Mixture Mycoplasma Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Arai, S.; Harasawa, R.; Ohno, T.; Takeuchi, M.; Hikizi, K.; Kobayashi, N.; Lee, I.; Kato, K.; Takahashi, H.; Okamoto, I.; Furudera, T. Comparative studies to detect mycoplasma contamination in bioindustrial materials, for validating standard method. Int. Org. Mycoplasmol. Lett. 3:48–49; 1994.Google Scholar
  2. 2.
    Barile, M. F.; Rottem, S. Mycoplasmas in cell cultures. In: Kahane, I.; Adoni, A., ed. Rapid diagnosis of mycoplasmas. New York: Plenum Press; 1993:155–193.Google Scholar
  3. 3.
    Berthold, W. M.; Grashoff, E.; Pfutzner, H.; Schubert, U.; Hauser, H. Detection of mycoplasma contaminations by the polymerase chain reaction. Cytotechnology 16:67–77; 1994.PubMedCrossRefGoogle Scholar
  4. 4.
    Boolske, G. Survey of mycoplasma infections in cell cultures and a comparison of detection methods. Zentralbl. Bakteriol. Mikrobiol. Hyg. 269:331–340; 1988.Google Scholar
  5. 5.
    Dussurget, O.; Roulland-Dussoix, D. Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera. Appl. Environ. Microbiol. 60:953–959; 1994.PubMedGoogle Scholar
  6. 6.
    Harasawa, R.; Mizusawa, H.; Nozama, T.; Asada, K.; Ito, I. Detection and tentative identification of dominant mycoplasma species in cell cultures by restriction analysis of the 16S–23S rRNA intergenic spacer regions. Res. Microbiol. 144:489–493; 1993.PubMedCrossRefGoogle Scholar
  7. 7.
    Harasawa, R. Nested PCR: application to the detection of mycoplasmas. In: Razin, S.; Tully, J. G., ed. Molecular and diagnostic procedures in mycoplasmology Vol. II. A4. London: Academic Press; 1995.Google Scholar
  8. 8.
    Hay, R. J.; Macy, M. L.; Chen, T. R. Mycoplasma infection of cultured cells. Nature 339:487–488; 1989.PubMedCrossRefGoogle Scholar
  9. 9.
    Hu, M.; Buck, C.; Jacobs, D.; Paulino, G.; Khouri, H. Application of PCR for detection and identification of mycoplasma contamination in virus stocks. In Vitro Cell. Dev. Biol. 31:710–715; 1995.Google Scholar
  10. 10.
    McGarrity, G. J.; Kotani, H. Cell culture mycoplasmas. In: Razin, S.; Barile, M., ed. The Mycoplasmas. Vol. 4. New York: Academic Press; 1985:353–390.Google Scholar
  11. 11.
    McGarrity, G. J.; Kotani, H. Cell culture mycoplasmas. In: Razin, S.; Barile, M., ed. The Mycoplasmas. Vol. 4. New York: Plenum Press; 1985:155–193.Google Scholar
  12. 12.
    McGarrity, G. J.; Kotoni, H.; Butler, G. H. Mycoplasmas and tissue culture cells. In: Maniloff, J.; McElhaney, R. N.; Finch, L. R.; Baseman, J. B., ed. Mycoplasmas, molecular biology and pathogenesis. Washington, D.C.: American Society for Microbiology; 1992:445–454.Google Scholar
  13. 13.
    Nakagawa, T.; Uemori, T.; Asada, K.; Kato, I.; Harasawa, R. Acholeplasma laidlawii has tRNA genes in the 16S–23S spacer of the rRNA operon. J. Bacteriol. 174:8163–8165; 1992.PubMedGoogle Scholar
  14. 14.
    Razin, S. DNA probes and PCR in diagnosis of mycoplasma infections. Mol. Cell. Probes 8:497–511; 1994.PubMedCrossRefGoogle Scholar
  15. 15.
    Rawadi, G.; Dussurget, O. Advances in PCR-based detection of mycoplasmas contaminating cell cultures. PCR Methods Appl. 4:199–208; 1995.PubMedGoogle Scholar
  16. 16.
    Uphoff, C. C.; Brauer, S.; Grunicke, D.; Gignac, S. M.; MacLeod, R. A. F.; Quentmeier, H.; Steube, K.; Tummler, M.; Vogas, M.; Wagner, B.; Drexler, H. G. Sensitivity and specificity of five different mycoplasma detection assays. Leukemia 6:335–341; 1992.PubMedGoogle Scholar

Copyright information

© Society for In Vitro Biology 1999

Authors and Affiliations

  • Jane Tang
    • 1
  • Mengdong Hu
    • 1
  • Sunmin Lee
    • 1
  • Richard Roblin
    • 1
  1. 1.American Type Culture CollectionManassas

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