Initial aspects of mineralization at the dentino-enamel junction in embryonic mouse incisor in vivo and in vitro: A tem comparative study

  • J. M. Meyer
  • P. Bodier-Houllé
  • F. J. G. Cuisinier
  • H. Lesot
  • J. V. Ruch
Growth, Differentiation And Senescence
Received:
Accepted:

Summary

The frontier between the enamel organ and the dental papilla, the future dentino-enamel junction, undergoes coordinated modifications. The mineralization of the extracellular matrix starts within the predentine, which is a prerequisite for the formation of the first enamel crystallites in vivo. We investigated the dentino-enamel junction using the embryonic mouse incisor as a model. Our data showed that the notion of the dentino-enamel junction should not be restricted to the thin interface classically described. A temporo-spatial survey from the epithelio-mesenchymal junction to the dentino-enamel junction delineated a clear sequence of events characterized by the early deposition of electron-dense granules, followed by the appearance of patches of stippled material at the dentino-enamel junction. The first tiny enamel crystallites appeared in the vicinity of this material which presented a well-ordered alignment. The comparison of data obtained in vivo on 17-, 18-, 19-d-old embryonic incisors with those obtained in vitro using 15-d-old embryonic incisors cultured for 7 d emphasizes the relevance of this sequence. Helicoidal growing crystals were observed in cultured tooth germs but never in vivo.

Key words

mouse incisor mineralization dentino-enamel junction 
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Copyright information

© Society for In Vitro Biology 1999

Authors and Affiliations

  • J. M. Meyer
    • 1
  • P. Bodier-Houllé
    • 1
  • F. J. G. Cuisinier
    • 1
  • H. Lesot
    • 1
  • J. V. Ruch
    • 1
  1. 1.Institut de Biologie Médicale—INSERM U 424 Faculté de MédecineStrasbourg CedexFrance

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