Effects of hepatocyte growth factor (HGF) and activin a on the morphogenesis of rat submandibular gland-derived epithelial cells in serum-free collagen gel culture

  • Miho Furue
  • Tetsuji Okamoto
  • Hiroyuki Hayashi
  • J. Denry Sato
  • Makoto Asashima
  • Shigeru Saito
Cellular Models

DOI: 10.1007/s11626-999-0014-8

Cite this article as:
Furue, M., Okamoto, T., Hayashi, H. et al. In Vitro Cell.Dev.Biol.-Animal (1999) 35: 131. doi:10.1007/s11626-999-0014-8

Summary

To study the mechanisms of morphogenesis in salivary gland regeneration, we have established the RSMG-1 cell line derived from submandibular gland (SMG) of 10-wk-old Wistar female rats in serum-free culture. Our finding that RSMG-1 cells originated from duct cells was based on morphology and immunohistochemical results. In three-dimensional serum-free collagen gel culture, HGF induced branching morphogenesis of RSMG-1 cells. Histological examination revealed that HGF-induced branching structure exhibited well-formed lumina. This morphology closely resembles that found in vivo. The cells also expressed activin A. Exogenously added activin A at a high concentration reduced HGF-induced branching morphogenesis. These findings suggest that the morphogenesis of the salivary gland is modulated by HGF and activin A. Our results show that the RSMG-1 cell line may be useful in studies of salivary gland regeneration.

Key words

salivary gland HGF activin branching morphogenesis 

Copyright information

© Society for In Vitro Biology 1999

Authors and Affiliations

  • Miho Furue
    • 1
  • Tetsuji Okamoto
    • 2
  • Hiroyuki Hayashi
    • 3
  • J. Denry Sato
    • 4
  • Makoto Asashima
    • 5
  • Shigeru Saito
    • 1
  1. 1.Department of Oral BiochemistryKanagawa Dental CollegeYokosuka City, KanagawaJapan
  2. 2.Department of Oral and Maxillofacial Surgery IHiroshima University School of DentistryHiroshimaJapan
  3. 3.Department of Oral HistochemistryKanagawa Dental CollegeUSA
  4. 4.Adirondack Biomedical Res. Inst.New York
  5. 5.Department of Life Sciences (Biology), Graduate School of Arts and SciencesThe University of TokyoTokyoJapan

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