Macrophage cell lines derived from major histocompatibility complex II-negative mice

  • Alison A. Beharka
  • Jason W. Armstrong
  • Stephen K. Chapes
Immunology

Summary

Two bone-marrow-derived macrophage cell lines, C2D and C2Dt, were isolated from major histocompatibility class II-negative knock-out mice. The C2D cell line was stabilized by continuous culture in colony-stimulating factor-1 and the C2Dt cell line was transformed with SV40 virus large T antigen. These cells exhibited phenotypic properties of macrophages including morphology and expression of Mac 1 and Mac 2 cell surface molecules. These cells also had comparable growth to the bone-marrow-derived macrophage cell line B6MP102. These new cell lines were not spontaneously cytotoxic and were only capable of modest killing of F5b tumor cells when stimulated with LPS and interferon-γ, but not when stimulated with LPS alone or with staphylococcal exotoxin. C2D and C2Dt cells phagocytosed labeled Staphylococcus aureus similarly to B6MP102 cells but less well than C2D peritoneal macrophages. These cell lines secreted interleukin-6, but not tumor necrosis factor or nitric oxide in response to LPS or staphylococcal enterotoxins A or B. C2Dt cells were tumorigenic in C2D and C57BL/6J mice but C2D cells were not. These data suggest that macrophage cell lines can be established from bone marrow cells of major histocompatibility complex II-negative mice.

Key words

bone-marrow-derived macrophages major histocompatibility class II staphylococcal exotoxins 

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Copyright information

© Society for In Vitro Biology 1998

Authors and Affiliations

  • Alison A. Beharka
    • 1
  • Jason W. Armstrong
    • 1
  • Stephen K. Chapes
    • 1
  1. 1.Division of BiologyKansas State UniversityManhattan

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