Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a NASA bioreactor
- 820 Downloads
Growth patterns of a number of human tumor cell lines that form three-dimensional structures of various architectures when cultured without carrier beads in a NASA rotary cell culture system are described and illustrated. The culture system, which was designed to mimic microgravity, maintained cells in suspension under very low-shear stress throughout culture. Spheroid (particulate) production occurred within a few hours after culture was started, and spheroids increased in size by cell division and fusion of small spheroids, usually stabilizing at a spheroid diameter of about 0.5 mm. Architecture of spheroids varied with cell type. Cellular interactions that occurred in spheroids resulted in conformation and shape changes of cells, and some cell lines produced complex, epithelial-like architectures. Expression of the cell adhesion molecules, CD44 and E cadherin, was upregulated in the three-dimensional constructs. Coculture of fibroblast spheroids with PC3 prostate cancer cells induced tenascin expression by the fibroblasts underlying the adherent prostate epithelial cells. Invasion of the fibroblast spheroids by the malignant epithelium was also demonstrated.
Key wordsspheroid architecture suspension culture low-shear culture
Unable to display preview. Download preview PDF.
- 1.Acker, H. Microenvironmental conditions in multicellular spheroids grown over liquid-overlay tissue culture conditions. In: Recent results in cancer research. Vol. 95. Berlin, Heidelberg: Springer-Verlag; 1984:116–133.Google Scholar
- 2.Bauer, K. D.; Keng, P.; Sutherland, R. M. Isolation of quiescent cells from multicellular tumor spheroids using centrifugal elutriation. Cancer Res. 42:3956–3965; 1980.Google Scholar
- 13.Kato, K.; Hibino, S.; Yagita, H., et al. Tenascin suppresses T-cell activation mediated by CD3 and costimulation molecules. Abs. #5085, 9th International Congress of Immunology, 23–29 July 1995, San Francisco, CA.Google Scholar
- 14.Laerum, O. D.; Bjerkrig, R. Monolayer and three-dimensional culture of rat and human central nervous system: normal and malignant cells and their interactions. Methods in neurosciences. Vol. 2. London: Academic Press; 1990:210–236.Google Scholar
- 21.Stamenkovic, I.; Aruffo, A.; Amiot, M., et al. The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells. EMBO 10:343–348; 1991.Google Scholar