A clonal stem cell line established from a mouse mammary placode with ability to generate functional mammary glands
The mammary gland develops from the placode at ectodermal invagination. The rudimentary parenchyma (mammary bud) develops mammary trees and alveolar structures, suggesting that the mammary bud consists of stem/progenitor cells. Here, we established a clonal stem cell line from a mammary bud of a p53 null female embryo at day 14.5. FP5-3-1 line was a homogeneous cell population with polygonal epithelial morphology and spontaneously became heterogeneous during passages. Recloning gave rise to four sublines; three sublines have basal epithelial property and one subline has luminal epithelial property. The former sublines generate functional mammary glands when injected into cleared fat pads and the latter subline does not. The cell lines also express many stemness-related genes. The clonal cell lines established in the present study are shown to be mammary stem cells and not tumorigenic. They provide useful models for normal and tumor biology of the mammary gland in vivo and in vitro.
KeywordsStem cell Clonal cell line Mammary gland p53 deficiency
The funding body had no role in the design or execution of the study.
This work was supported by a Grants for Support of the Promotion of Research at Tokyo University of Science (to YT and TN).
- Ginestier C, Hur MH, Charafe-Jauffret E, Monville F, Dutcher J, Brown M, Jacquemier J, Viens P, Kleer CG, Liu S, Schott A, Hayes D, Birnbaum D, Wicha MS, Dontu G (2007) ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell 1:555–567CrossRefGoogle Scholar
- Umezu T, Hanazono M, Aizawa S, Tomooka Y (2003) Characterization of newly established clonal oviductal cell lines and differentiation of gene expression. In Vitro Cell Biol Anim 39:146–156Google Scholar