Next-generation cell lines established from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)
The fall armyworm, Spodoptera frugiperda (Sf), is a polyphagous lepidopteran herbivore that consumes more than 80 plant species, including many economically important crops, such as corn, soybeans, and sorghum. While already a serious pest in the Americas, it was recently introduced into Africa, India, and China. Because of its high economic costs in the New World and the continent-wide damage potentials in Africa, research to develop advanced pest management technologies is necessary. We are supporting this need by developing novel, next-generation insect cell lines from targeted tissues. Cell lines, such as these, will boost insecticide discovery programs and lead to innovative pest management solutions. Here, we report on the establishment of 16 new cell lines from larval S. frugiperda tissues: nine from the central nervous system, three from the aorta, and four from the testes. We confirmed the identities of the cell lines by DNA amplification fingerprinting polymerase chain reaction, determined their doubling times from growth curves, and described cell types via microscopy. We also developed 16 sublines from three neuronal cell lines.
KeywordsInsect cell line Lepidoptera Fall armyworm Spodoptera frugiperda DAF-PCR Agriculture
Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the USDA are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. We acknowledge the support of BAYER AG through Cooperative Research Agreement 58-3K95-5-1728.
- Blanco CA, Portilla M, Jurat-Fuentes JL, Sánchez JF, Viteri D, Vega-Aquino P, Terán-Vargas AP, Azuara-Domínguez A, López JD Jr, Arias R, Zhu Y-C, Lugo-Barrera D, Jackson R (2010) Susceptibility of isofamilies of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Cry1Ac and Cry1Fa proteins of Bacillus thuringiensis. Southwest Entomol 35:409–415CrossRefGoogle Scholar
- Boeckel GR, Ehrlich BE (2018) NCS-1 is a regulator of calcium signaling in health and disease. BBA-Mol Cell Res 1865:1660–1667Google Scholar
- Casmuz A, Juarez ML, Socias MG, Murua G, Prieto S, Medina S, Willink E, Gastaminza G et al (2010) Revisión de los hospederos del gusano cogollero del maíz, Spodoptera frugiperda (Lepidoptera: Noctuidae). Rev Soc Entomol Argent (En línea) Revista de la Sociedad Entomológica Argentina 69:209–231Google Scholar
- Claus JD, Gioria VV, Micheloud GA, Visnovsky G (2012) Production of insecticidal baculoviruses in insect cell cultures: potential and limitations. Insecticides-basic and other applications. www.intechopen.com. Accessed 21 June 2018
- Jeger M, Bragard C, Caffier D, Candresse T, Chatzivassiliou E, Dehnen-Schmutz K, Gilioli G, Gregoire J-C, Miret JAJ, Navarro MN, Niere B, Parnell S, Potting R, Rafoss T, Rossi V, Urek G, Van Bruggen A, Van der Werf W, West J, Winter S, Gardi C, Aukhojee M, MacLeod A (2017) Pest categorisation of Spodoptera frugiperda. EFSA Journal 15(7):4927Google Scholar
- León-García I, Rodríguez-Leyva E, Ortega-Arenas LD, Solís-Aguilar JF (2012) Susceptibilidad de Spodoptera frugiperda (JE Smith)(Lepidoptera: Noctuidae) a insecticidas asociada a césped en Quintana Roo, México. Agrociencia 46:279–287Google Scholar