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LncRNA TUG1 contributes to cardiac hypertrophy via regulating miR-29b-3p

  • Xue Zou
  • Jialiang Wang
  • Li Tang
  • Qian WenEmail author
Article
  • 26 Downloads

Abstract

Cardiac hypertrophy with maladjusted cardiac remodeling is the leading cause of heart failure. In the past decades, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have been proved to exert multiple functions in cellular biological behaviors; however, their role in cardiac hypertrophy remains largely unclear. Presently, we first obtained hypertrophic H9c2 cells by treating with angiotensin II (Ang II) and uncovered upregulation of lncRNA taurine upregulated gene 1 (TUG1) in such H9c2 cells. Then, we demonstrated that silencing TUG1 attenuated Ang II–induced cardiac hypertrophy. Besides, a strong interactivity of TUG1 with miR-29b-3p at the putative sites was validated, suggesting that TUG1 was an endogenous sponge of miR-29b-3p in H9c2 cells. Additionally, the expression of miR-29b-3p was strikingly reduced by TUG1 upregulation and also inhibited under Ang II treatment, whereas it was restored after silencing TUG1 in hypertrophic cells. Also, we proved miR-29b-3p as a negative regulator in cardiac hypertrophy. Finally, miR-29b-3p inhibition abolished the anti-hypertrophy effect of TUG1 depletion in Ang II–treated H9c2 cells. Collectively, our findings confirmed that TUG1 functioned as a positive modulator of cardiac hypertrophy via sponging miR-29b-3p, indicating that TUG1 might serve as a potential target for the treatment of cardiac hypertrophy and even heart failure.

Keywords

TUG1 miR-29b-3p Ang II Cardiac hypertrophy H9c2 cells 

Notes

Acknowledgements

Thank you for all the participators.

Funding

This work was funded by the role of GRK4 methylation in the hypertension induced by pregnancy cold-exposure.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Department of CardiologyDaping Hospital, The Third Military Medical UniversityChongqingPeople’s Republic of China

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