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Simulated microgravity inhibits the viability and migration of glioma via FAK/RhoA/Rock and FAK/Nek2 signaling

  • Banglian Deng
  • Rongrong Liu
  • Xin Tian
  • Zucheng HanEmail author
  • Jun ChenEmail author
Article
  • 89 Downloads

Abstract

Due to excessive proliferation and metastasis, glioma is the most common primary tumor in the central nervous system. Previous reports show simulated microgravity (SMG) has the ability to inhibit the proliferation and migration of cancer. The aim of this study was to evaluate the viability and migration of U251 cells in SMG environment. SMG induced apoptosis of U251 cells. The FAK/RhoA/Rock and FAK/Nek2 signaling events were attenuated by SMG to destabilize actin cytoskeleton and centrosome disjunction, which caused G2/M arrest and inhibition of cell viability and migration. Overexpressed FAK reversed SMG-induced inhibition of viability and migration in U251 cells, which increased downstream RhoA/Rock signaling and Nek2. These findings reveal novel pathways of FAK/RhoA/Rock and FAK/Nek2 are affected by SMG, and highlight an opportunity to expand therapeutic options in a variety of settings.

Keywords

Glioma Simulated microgravity FAK RhoA/Rock Nek2 

Notes

Acknowledgements

We thank Dr. Austin Cape at ASJ Editors for careful reading and feedback.

Funding information

The present study was supported by the Youth Science and Technology Innovation Personnel Training Project of Shaanxi Province (No. 2017KJXX-84), the National Natural Science Foundation of China (Nos. 81402055 and 81602494), and the Natural Science Foundation of Shaanxi Province (No. 2016JM8014).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11626_2019_334_MOESM1_ESM.docx (212 kb)
ESM 1 (DOCX 211 kb)

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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Department of Oral Implant, Stat Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, School of StomatologyFourth Military Medical UniversityXi’anChina
  2. 2.Lian Bang Dental Hospital of Xin Cheng DistrictXi’anChina
  3. 3.Department of Microbiology, School of Basic MedicineFourth Military Medical UniversityXi’anChina
  4. 4.Department of CardiologyTraditional Chinese Medicine Hospital of Shaanxi ProvinceXi’anChina
  5. 5.Department of EncephalopathyTraditional Chinese Medicine Hospital of Shaanxi ProvinceXi’anChina

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