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miR-338-3p regulates osteoclastogenesis via targeting IKKβ gene

  • Dequn Niu
  • Zheng Gong
  • Xuemin Sun
  • Jianchang Yuan
  • Tiantian Zheng
  • Xun Wang
  • Xu Fan
  • Yingji Mao
  • Xianfu Liu
  • Baoding Tang
  • Yingxiao FuEmail author
Article
  • 65 Downloads

Abstract

This study determined the effects of miR-338-3p on osteoclast (OC) differentiation and activation. The change levels of miR-338-3p in differentiated OCs were investigated by microRNA microarray assay and quantitative real-time PCR analysis. The effects of miR-338-3p on the differentiation and activation of OCs were determined by tartrate-resistant acid phosphatase staining resorption activity assay and Western blot. Target genes of miR-338-3p were identified by target gene prediction and dual-luciferase reporter gene detection assay as well as Western blot. Results showed that miR-338-3p was markedly downregulated in differentiated OCs. miR-338-3p could inhibit the formation and absorption activity of OCs. Western blot showed that miR-338-3p could influence the change levels of OC differentiation–related proteins. Dual-luciferase reporter gene detection assay and Western blot both showed that miR-338-3p directly targeted IKKβ gene. In conclusion, miR-338-3p may affect the formation and activity of OCs by targeting the IKKβ gene.

Keywords

miR-338-3p Osteoclastogenesis IKKβ gene 

Notes

Funding information

This study was supported by the Project of Natural Science Foundation of Anhui Province (1508085QH172), the Natural Science Research Project of Universities in Anhui (KJ2017A225, KJ2018A1011, and KJ2017A237), the Natural Science Foundation of Bengbu Medical College (BYKY1614ZD), and the National Training Programs of Innovation and Entrepreneurship for Undergraduate (201410367029, 201610367004, 201810367019).

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Supplementary material

11626_2019_325_MOESM1_ESM.xls (973 kb)
ESM 1 (XLS 973 kb)
11626_2019_325_MOESM2_ESM.xlsx (35 kb)
ESM 2 (XLSX 35 kb)

References

  1. Abu-Amer Y (2013) NF-kappaB signaling and bone resorption. Osteoporos Int 24:2377–2386CrossRefGoogle Scholar
  2. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297CrossRefGoogle Scholar
  3. Chen C, Cheng P, Xie H, Zhou HD, Wu XP, Liao EY, Luo XH (2014) MiR-503 regulates osteoclastogenesis via targeting RANK. J Bone Miner Res 29:338–347CrossRefGoogle Scholar
  4. Detsch R, Boccaccini AR (2015) The role of osteoclasts in bone tissue engineering. J Tissue Eng Regen Med 9:1133–1149CrossRefGoogle Scholar
  5. Dole NS, Delany AM (2016) MicroRNA variants as genetic determinants of bone mass. Bone 84:57–68CrossRefGoogle Scholar
  6. Horne WC, Duong L, Sanjay A, Baron R (2008) Regulating bone resorption: targeting Integrins, calcitonin receptor, and cathepsin K, Principles of Bone Biology, 3rd edn. Academic Press, pp 221–236Google Scholar
  7. Iotsova V, Caamano J, Loy J, Yang Y, Lewin A, Bravo R (1997) Osteopetrosis in mice lacking NF-κB1 and NF-κB2. Nat Med 3:1285–1289CrossRefGoogle Scholar
  8. Khandaker M, Riahinezhad S, Sultana F, Vaughan MB, Knight J, Morris TL (2016) Peen treatment on a titanium implant: effect of roughness, osteoblast cell functions, and bonding with bone cement. Int J Nanomed 11:585–595CrossRefGoogle Scholar
  9. Kim JH, Kim N (2016) Signaling pathways in osteoclast differentiation. Chonnam Med J 52:12–17CrossRefGoogle Scholar
  10. Liu H, Sun Q, Wan C, Li L, Zhang L, Chen Z (2014) MicroRNA-338-3p regulates osteogenic differentiation of mouse bone marrow stromal stem cells by targeting Runx2 and Fgfr2. J Cell Physiol 229:1494–1502CrossRefGoogle Scholar
  11. Ono T, Nakashima T (2018) Recent advances in osteoclast biology. Histochem Cell Biol 149:325–341CrossRefGoogle Scholar
  12. Otero JE, Dai S, Alhawagri MA, Darwech I, Abu-Amer Y (2010) IKKbeta activation is sufficient for RANK-independent osteoclast differentiation and osteolysis. J Bone Miner Res 25:1282–1294CrossRefGoogle Scholar
  13. Shigeru K, Toru Y, Manabu K, Yuki N (2012) Human receptor activator of NF-κB ligand (RANKL) induces osteoclastogenesis of primates in vitro. In Vitro Cell Dev Biol Anim 48:593–598CrossRefGoogle Scholar
  14. Soo-Jin K, So-Young K, Hyun-Hee S, Hye-Seon C (2005) Sulforaphane inhibits osteoclastogenesis by inhibiting nuclear factor-κ B. Mol Cells 20:364–370Google Scholar
  15. Tang P, Xiong Q, Ge W, Zhang L (2014) The role of microRNAs in osteoclasts and osteoporosis. RNA Biol 11:1355–1363CrossRefGoogle Scholar
  16. Xia Z, Chen C, Chen P, Xie H, Luo X (2011) MicroRNAs and their roles in osteoclast differentiation. Front Med 5:414–419CrossRefGoogle Scholar
  17. Yong X, Lihai Z, Yanpan G, Wei G, Peifu T (2015) The Multiple Roles of Microrna-223 in Regulating Bone Metabolism. Molecules 20:19433–19448Google Scholar
  18. Zhang XH, Geng GL, Su B, Liang CP, Wang F, Bao JC (2016) MicroRNA-338-3p inhibits glucocorticoid-induced osteoclast formation through RANKL targeting. Genet Mol Res 15:1–9Google Scholar
  19. Zhao QX, Wang X, Liu Y, He A, Jia R (2010) NFATc1: functions in osteoclasts. Int J Biochem Cell Biol 42:576–579CrossRefGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  • Dequn Niu
    • 1
  • Zheng Gong
    • 2
  • Xuemin Sun
    • 3
  • Jianchang Yuan
    • 2
  • Tiantian Zheng
    • 2
  • Xun Wang
    • 2
  • Xu Fan
    • 2
  • Yingji Mao
    • 2
  • Xianfu Liu
    • 4
  • Baoding Tang
    • 2
  • Yingxiao Fu
    • 2
    Email author
  1. 1.Department of Gynaecology and ObstetricsThe Second Affiliated Hospital of Bengbu Medical CollegeBengbuPeople’s Republic of China
  2. 2.Department of BioscienceBengbu Medical CollegeBengbuPeople’s Republic of China
  3. 3.Department of Clinical MedicineBengbu Medical CollegeBengbuPeople’s Republic of China
  4. 4.Department of Surgical OncologyThe First Affiliated Hospital of Bengbu Medical CollegeBengbuPeople’s Republic of China

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