Retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) restore the germline competence of in vitro cultured chicken blastodermal cells
Chicken blastodermal cells (BCs) are pluripotent stem cells derived from early embryos and may be easily obtained and manipulated. However, in vitro cultured BCs have extremely low germline capacity, which may limit their applications. Research on the germ cell differentiation of mammalian pluripotent cells using chemical-inducing agents has gained popularity, and tremendous achievements have been made. Whether chemical-inducing agents allow acquirement of germline competence in BCs is, however, questionable. In this study, retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted the expression of germline-specific genes and restored the germline competence of in vitro cultured BCs. Moreover, BCs induced with RA and BMP4 could efficiently produce gonadal chimeric chick embryos. These results may greatly enhance the potential applications of BCs in biotechnology and basic research.
KeywordsChicken blastodermal cells Retinoic acid Bone morphogenetic protein 4 Germline competence Genetically modified chicken
We thank Zhiqiang Guan for the help in the experiment.
This study was supported by Technical Innovation to Guide Specialy of Guangxi Province (2017AD10038) and the National Natural Science Foundation of China (31460644).
Compliance with ethical standards
All experiments were in accordance with the guidelines of the regional Animal Ethics Committee, and the Institutional Animal Care and Use Committee of Guangxi University approved all experiments.
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