Human menstrual blood–derived stem cells protect H9c2 cells against hydrogen peroxide–associated apoptosis
- 81 Downloads
Human menstrual blood–derived mesenchymal stem cells (MenSCs) hold great promise for regenerative medicine. Here, H2O2-associated damage in H9c2 cells was employed as an in vitro ischemia–reperfusion model, and the transwell system was used to explore the beneficial effects of MenSCs on the H2O2-induced damage of myocardial H9c2 cells. H2O2 treatment resulted in decreased viability and migration rate, with increased apoptosis levels in cells. By contrast, upon co-culture with MenSCs, H9c2 cell viability and migration were increased, whereas the apoptotic rate decreased. Additionally, western blot and qRT-PCR showed that MenSCs mediated the anti-apoptotic role by downregulating the pro-apoptotic genes Bax and caspase-3, while upregulating the anti-apoptotic effector Bcl-2. Furthermore, co-culture with MenSCs resulted in elevated expression of N-cadherin after H2O2 treatment. These findings indicate that MenSCs protect H9c2 cells against H2O2-associated programmed cell death and would help develop therapeutic tools for cardiomyocyte apoptosis associated with oxidative stress.
KeywordsMenstrual blood–derived mesenchymal stem cell H9c2 cell Apoptosis Myocardial ischemia–reperfusion injury
This work was supported by Nantong Science and Technology Project (MS22016030), the National Natural Science Foundation of China (81501189), and Jiangsu Government Scholarship for Overseas Studies (JS-2016-061).
Compliance with ethical standards
All procedures were performed after informed consent provision by the volunteers, with approval from the Ethics Committee of Nantong Maternal and Child Health Care Hospital, affiliated to Nantong University, China (license no. is 2016-023).
- Fatkhudinov T, Bolshakova G, Arutyunyan I, Elchaninov A, Makarov A, Kananykhina E, Khokhlova O, Murashev A, Glinkina V, Goldshtein D (2015) Bone marrow-derived multipotent stromal cells promote myocardial fibrosis and reverse remodeling of the left ventricle. Stem Cells Int 2015:746873CrossRefGoogle Scholar
- Kovecsi A, Gurzu S, Szentirmay Z, Kovacs Z, Bara TJ, Jung I (2017) Paradoxical expression pattern of the epithelial mesenchymal transition-related biomarkers CD44, SLUG, N-cadherin and VSIG1/glycoprotein A34 in gastrointestinal stromal tumors. World J Gastrointest Oncol 9:436–443CrossRefGoogle Scholar
- Zhu GJ, Song PP, Zhou H, Shen XH, Wang JG, Ma XF, Gu YJ, Liu DD, Feng AN, Qian XY (2018) Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, beta-catenin and ZEB2 in laryngeal squamous cell carcinoma. Oncol Lett 15:3472–3481Google Scholar