Effects of lithium on the secretory production of recombinant antibody from insect cells

  • Yuki Ohmuro-Matsuyama
  • Tomohisa Katsuda
  • Hideki YamajiEmail author


Monoclonal antibodies and antibody fragments are widely used in therapeutics and diagnoses. While mammalian cells serve as the host cells for antibody production, insect cells can produce large quantities of secretory antibodies in serum-free suspension cultures. The effects of lithium on the processes of autophagy and apoptosis in mammalian cells are well chronicled. In the present study, stably transformed insect cells, which produce an engineered antibody molecule, were cultured with lithium chloride in a serum-free medium. Treatment with lithium chloride induced autophagy and apoptosis in recombinant insect cells and led to increases in the yields of secreted antibodies.


Insect cell culture Recombinant protein production Lithium chloride High Five cells Antibody 



This research was partially supported by the developing key technologies for discovering and manufacturing pharmaceuticals used for next-generation treatments and diagnoses both from the Ministry of Economy, Trade and Industry, Japan (METI) and from the Japan Agency for Medical Research and Development (AMED).

Supplementary material

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Copyright information

© The Society for In Vitro Biology 2018

Authors and Affiliations

  1. 1.Department of Chemical Science and Engineering, Graduate School of EngineeringKobe UniversityKobeJapan
  2. 2.Laboratory for Chemistry and Life Science, Institute for Innovative ResearchTokyo Institute of TechnologyYokohamaJapan
  3. 3.Manufacturing Technology Association of Biologics (MAB)c/o Integrated Research Center of Kobe UniversityKobeJapan

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