Establishment, characterization, and toxicological application of a spontaneous immortalized cell line from the striped field mouse, Apodemus agrarius
- 5 Downloads
It is important to secure various biological resources in situations of diminishing wildlife genetic diversity. Cultured cells are useful bioresources because they can stably store genetic information for a long time and can be expanded efficiently. Here, we established fibroblast cell lines from Apodemus agrarius as a new living resource. A. agrarius is an important sub-predator species in ecosystem food chains and for the study of infection epidemiology. Established cell lines were characterized by chromosome and mitochondrial gene analysis, the observation of cell morphology, and their anchorage-dependent growth pattern. We also examined susceptibility to endocrine disruptors (EDCs), which threaten biodiversity, using these established cell lines. Nonylphenol (NP) is a well-known EDC that threatens wildlife; however, its impact is poorly understood. Sensitivity to NP was confirmed based on two cell viability assays, namely MTT and lactate dehydrogenase. Cells exposed to NP were analyzed for abnormalities in cell growth and mitochondrial function by evaluating the expression of genes (specifically, those encoding growth hormone receptor and cytochrome C oxidase). This newly established cell line represents a valuable tool for the evaluation of toxic substances such as EDCs and this cell was biobanked for study about relationship between various environmental pollution and decreasing biodiversity.
KeywordsBioresource Apodemus agrarius Immortalized cell line Endocrine disruptors Nonylphenol
This work was supported by the National Institute of Biological Resources (NIBR) [grant numbers NIBR201526201], funded by the Ministry of Environment (MOE), Republic of Korea, and the Research Institute for Veterinary Science and the BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Borowicz S, Van Scoyk M, Avasarala S, Rathinam MKK, Tauler J, Bikkavilli RK, Winn RA (2014) The soft agar colony formation assay. J Visual ExpGoogle Scholar
- Hanson AM, Ickstadt AT, Marquart DJ, Kittilson JD, Sheridan MA (2017) Environmental estrogens inhibit mrna and functional expression of growth hormone receptors as well as growth hormone signaling pathways in vitro in rainbow trout (oncorhynchus mykiss). Gen Comp Endocrinol 246:120–128CrossRefGoogle Scholar
- Lei F, Zu-guo W, Yao Y, Xiang-ming X, Pin-qiang G (2015) Population change of farmland rodent and the influences of climate and cultivation factors in fengxian district of Shanghai, China. Yingyong Shengtai Xuebao 26Google Scholar
- Nooteboom M, Johnson R, Taylor RW, Wright NA, Lightowlers RN, Kirkwood TB, Mathers JC, Turnbull DM, Greaves LC (2010) Age-associated mitochondrial DNA mutations lead to small but significant changes in cell proliferation and apoptosis in human colonic crypts. Aging Cell 9:96–99CrossRefGoogle Scholar