In Vitro Cellular & Developmental Biology - Animal

, Volume 53, Issue 8, pp 744–751 | Cite as

The influence of simulated microgravity on proliferation and apoptosis in U251 glioma cells

  • Jiao Zhao
  • He Ma
  • Leitao Wu
  • Liang Cao
  • Qianqian Yang
  • Haijun Dong
  • Zongren Wang
  • Jing MaEmail author
  • Zhen LiEmail author


Several studies have indicated that microgravity can influence cellular progression, proliferation, and apoptosis in tumor cell lines. In this study, we observed that simulated microgravity inhibited proliferation and induced apoptosis in U251 malignant glioma (U251MG) cells. Furthermore, expression of the apoptosis-associated proteins, p21 and insulin-like growth factor binding protein-2 (IGFBP-2), was upregulated and downregulated, respectively, following exposure to simulated microgravity. These findings indicate that simulated microgravity inhibits proliferation while inducing apoptosis of U251MG cells. The associated effects appear to be mediated by inhibition of IGFBP-2 expression and stimulation of p21 expression. This suggests that simulated microgravity might represent a promising method to discover new targets for glioma therapeutic strategy.


Simulated microgravity Malignant glioma Apoptosis P21 IGFBP-2 



This work was supported by the National Natural Science Foundation of China (No. 81273879). We thank Dr. Wen-dong Bai, Dr. Chu-chao Zhu, Dr. Yuan Xing, and Dr. Lu Wang for their expert technical assistance.


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Copyright information

© The Society for In Vitro Biology 2017

Authors and Affiliations

  • Jiao Zhao
    • 1
  • He Ma
    • 2
  • Leitao Wu
    • 1
  • Liang Cao
    • 1
  • Qianqian Yang
    • 2
  • Haijun Dong
    • 1
  • Zongren Wang
    • 1
  • Jing Ma
    • 1
    Email author
  • Zhen Li
    • 2
    Email author
  1. 1.Department of Traditional Chinese Medicine, Xijing HospitalFourth Military Medical UniversityXi’anChina
  2. 2.Department of Histology and EmbryologyFourth Military Medical UniversityXi’anChina

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