A cell clone strain from Mythimna separata (Lepidoptera: Noctuidae) highly susceptible to Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and M. separata NPV (MsNPV)

  • Xiang-Qian Meng
  • Gui-Ling Zheng
  • Chuan-De Zhao
  • Fang-Hao Wan
  • Chang-You Li
Article
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Abstract

In this study, we describe a cell line, Ms-10C, cloned from the line QAU-Ms-E-10 (simplified Ms-10), an embryonic line from Mythimna separata. The cloned cell line was significantly more sensitive to nucleopolyhedrovirus (NPV). Ms-10C cells were mainly spherical with a diameter of 14.42 ± 2.23 μm. DNA amplification fingerprinting (DAF) confirmed the profile of PCR-amplified bands of the cloned cell line was consistent with those of the parental cell line, Ms-10. The sequencing result of the mitochondrial cytochrome c oxidase I (mtCO I) fragment confirmed that the amplified 636-bps mtCOI fragment was 100% identical to that of M. separata. Its chromosomes exhibited the typical characters of lepidopteran cell lines. Its population doubling time was 42.2 h at 27°C. Ms-10C was more sensitive than Ms-10 to both Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and M. separata nucleopolyhedrovirus (MsNPV). At 4 d post infection, the infection rates of two viruses reached 94.2 and 92.3%, respectively. The availability of this cell clone strain will provide a useful tool for the basic research on nucleopolyhedrovirus and for potential application in expression of recombinant proteins with baculovirus expression vector system.

Keywords

Mythimna separata Cell clone strain Autographa californica multiple nucleopolyhedrovirus (AcMNPV) Mythimna separata nucleopolyhedrovirus (MsNPV) 
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Notes

Acknowledgements

This study was supported by the State Key Laboratory of Integrated Management of Pest Insects and Rodents (Grant No. ChineseIPM1602), Key Research and Development Program in Shandong Province (2015GNC111003), and Wheat Industrial I & E Team of Qingdao Modern Agriculture Industrial Technology System (QDAIET-16-01-06).

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Copyright information

© The Society for In Vitro Biology 2017

Authors and Affiliations

  1. 1.Key Lab of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant ProtectionQingdao Agricultural UniversityQingdaoChina
  2. 2.State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of ZoologyChinese Academy of SciencesBeijingChina
  3. 3.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina

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