Interaction of silver and gold nanoparticles in mammalian cancer: as real topical bullet for wound healing— A comparative study
The present study evaluates in vitro cytotoxic effects and the mode of interaction of biologically synthesized Ag and Au nanoparticles (NPs) using Brassica oleracea L. var. capitata f. rubra (BOL) against HT-1080 cancer cells and bacterial cells as well as their wound healing efficacy using a mouse model. UV–visible spectroscopy, scanning electron microscopy, high-resolution transmission electron microscopy, and energy-dispersive X-ray analysis have ascertained the formation of nano-sized Ag and Au particles. Fourier transform infrared analysis has confirmed that polyphenol and amide groups in BOL act as capping as well as reducing agents. The free radical scavenging activity under in vitro conditions is found to be higher for the Ag NPs when compared to the Au NPs. Acridine orange–ethidium bromide dual staining and comet assay have indicated that the cytotoxic effects are mediated through nuclear morphological changes and DNA damage. The intracellular localization of Ag and Au NPs in HT-1080 cells and their subsequent effect on apoptosis and necrosis were analyzed by flow cytometry while the mode of interaction was established by scanning electron microscopy under field emission mode and by bio-transmission electron microscopy. These methods of analysis clearly revealed that the Ag and Au NPs have easily entered and accumulated into the cytosol and nucleus, resulting in activation of inflammatory and apoptosis pathways, which in turn cause damage in DNA. Further, mRNA and protein expression of caspase-3 and caspase-7, TNF-α, and NF-κB have provided sufficient clues for induction of intrinsic and extrinsic apoptosis and inflammatory pathways in Ag NP- and Au NP-treated cells. Evaluation of wound healing properties of Ag and Au NPs using a mouse model indicates rapid healing of wounds. In addition, no clear toxic effects and no nuclear abnormalities in peripheral blood cells are observed. Ag NPs appear to be a better anticancer therapeutic agent than Au NPs. Nonetheless, both Ag NPs and Au NPs show potential for promoting topical wound healing without any toxic effects.
KeywordsSilver Gold Nanoparticles Apoptosis Cancer Wound healing Inflammation Peripheral blood
The TEM and SEM samples were analyzed using the JEM-2010 (JEOL) installed at the Center for University-Wide Research Facilities (CURF) at Chonbuk National University. We thank Mrs. Eun-Jin Choi at the CURF at Chonbuk National University.
Compliance with ethical standards
All procedures relating to the animals and their care conformed to the international guideline “Principles of Laboratory Animals Care” (NIH Publication No. 85-23, revised 1985).
- Ahmed MF, Rao A (2014) Simultaneous determination of phenolic compounds in Brassica oleracea L. var capitata. by high-performance liquid chromatography. Int J Pharm Pharm Sci 6:534Google Scholar
- Jeyaraj M, Rajesh M, Arun R, MubarakAli D, Sathishkumar G, Sivanandhan G, Dev GK, Manickavasagam M, Premkumar K, Thajuddin N, Ganapathi A (2013a) An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells. Colloids Surf B Biointerfaces 102:708CrossRefPubMedGoogle Scholar
- Kulandaivelu B, Gothandam KM (2016) Cytotoxic effect on cancerous cell lines by biologically synthesized silver nanoparticles. Braz Arch Boil technol 59:1Google Scholar
- Pardee AB, Stein GS (2011) The biology and treatment of cancer: understanding cancer. Wiley, HobokenGoogle Scholar
- Ramar M, Manikandan B, Marimuthu PN, Raman T, Mahalingam A, Subramanian P, Karthick S, Munusamy A (2015) Synthesis of silver nanoparticles using Solanum trilobatum fruits extract and its antibacterial, cytotoxic activity against human breast cancer cell line MCF 7. Spectro chimica Acta A 140:223CrossRefGoogle Scholar
- Salunke GR, Ghosh S, Kumar RS, Khade S, Vashisth P, Kale T, Chopade S, Pruthi V, Kundu G, Bellare JR, Chopade BA (2014) Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control. Int J Nanomedicine 9:2635PubMedPubMedCentralGoogle Scholar
- Sharma G, Sharma AR, Kurian M, Bhavesh R, Nam JS, Lee SS (2014) Green synthesis of silver nanoparticle using Myristica fragrans (nutmeg) seed extract and its biological activity. Dig J Nanomater Biostruct 9:325Google Scholar
- Woolfenden BS, Wilson RL (1982) Radical cations as reference chromogens in studies of one-electron transfer reactions: pulse radiolysis studies of 2, 2-azinobis-(3-ethylbenzthiazoline-6-sulfonate). J Chem Soc Perkin Trans:805–812Google Scholar