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Impact of prolonged oocyte incubation time before vitrification on oocyte survival, embryo formation, and embryo quality in mice

  • Azade Karami
  • Mitra BakhtiariEmail author
  • Mehri Azadbakht
  • Rostam Ghorbani
  • Mozafar Khazaei
  • Mansour Rezaei
Article

Abstract

Oocyte incubation time before freezing is one of the factors affecting oocyte vitrification. In the assisted reproductive technology (ART) clinics, it is sometimes decided to perform oocyte vitrification after a long period of incubation time due to various conditions, such as inability to collect semen samples, unsuccessful urological interventions (PESA, TESE, etc.), or unexpected conditions. A time factor of up to 6 h has been studied in the available reports. Therefore, this study was designed to evaluate oocyte incubation time before freezing at 0, 6, 12, 18, and 24 h after retrieval. Metaphase II (MII) oocytes were obtained from NMRI female mice after being randomly divided into the five groups of 0, 6, 12, 18, and 24 h of freezing via hormonal stimulation following retrieval and entered into the vitrification-warming process. The thawed oocytes were evaluated according to the survival criteria and then inseminated with the sperms of male mice for in vitro fertilization. The next day, the embryo formation rate and embryo quality were assessed. Our results demonstrated that even after 24 h of incubation, the survival rate of oocytes was 51.35% with the embryo formation rate of 73.21%. However, the survival and embryo formation rates significantly decreased within 12, 18, and 24 h after retrieval compared to the groups vitrified at 0 h. The embryo quality was significantly reduced by vitrification at 0 to 24 h after retrieval. According to our data, although a prolonged incubation time before freezing reduced the survival rate, there was still a chance for oocytes to stay alive with acceptable embryo formation and quality rates after vitrification warming of oocytes.

Keywords

Oocyte vitrification Prolonged incubation time Survival Embryo formation Embryo quality 

Notes

Acknowledgments

This study was supported by Kermanshah University of Medical Sciences School of Medicine. We should warmly appreciate the valuable contribution of those responsible in the Histology-Embryology Laboratory of the Department of Biology, Faculty of Sciences, Razi University, Kermanshah. The animals were taken care of according to the institutional guidelines of Kermanshah University of Medical Sciences and the National Institutes of Health (NIH) guidelines, including a 12/12 light/dark cycle, a temperature of 25°C, and free access to food and water.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© The Society for In Vitro Biology 2017

Authors and Affiliations

  • Azade Karami
    • 1
  • Mitra Bakhtiari
    • 2
    Email author
  • Mehri Azadbakht
    • 3
  • Rostam Ghorbani
    • 2
  • Mozafar Khazaei
    • 2
  • Mansour Rezaei
    • 4
  1. 1.Students Research CommitteeKermanshah University of Medical SciencesKermanshahIran
  2. 2.Fertility & Infertility Research CenterKermanshah University of Medical SciencesKermanshahIran
  3. 3.Department of Biology, Faculty of SciencesRazi UniversityKermanshahIran
  4. 4.Department of Biostatistics & EpidemiologyKermanshah University of Medical SciencesKermanshahIran

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