Mitochondrial membrane potential (MMP) regulates sperm motility
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Sperm motility is the major decisive factor in determining male fertility. The objective of the present study was to analyse the effect of mitochondrial membrane potential (MMP) on the temporal regulation of sperm motility. Observations were recorded in various rodent species and among differentially motile sperm fractions including swim up and leftover layer of human semen sample using JC-1 stain (a marker of the MMP) through FACS. Swim-up sperms having highest motility showed significantly higher MMP as compared to leftover sperms, which had the least motility. Interestingly, infertile patients with compromised motility showed low MMP as compared to the healthy individuals. Further, as per the time lapse, sperm motility goes down, at the same time, it was observed that MMP also decreases in human as well as in rodent sperms. Treatment of known spermicides on human sperms reduced their motility drastically which in turn also reduced its MMP significantly. Treatment of human sperms with oxidative uncoupler also impeded their motility by reducing MMP, indicating a definitive role on MMP on sperm motility and fertility. Based on the results of the study, MMP can be considered as a potential regulator and indicator of sperm motility and hence could be directly related to male fertility.
KeywordsMitochondrial membrane potential Spermatozoa Sperm motility Infertility
This research work was financially supported by PROGRAM, Council of Scientific and Industrial Research (CSIR), New Delhi, Government of India. We thank CSIR—Central Drug Research Institute, Lucknow as well as the central FACS facility of the institute.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- Barroso G, Taylor S, Morshedi M, Manzur F, Gavino F, Oehninger S (2006) Mitochondrial membrane potential integrity and plasma membrane translocation of phosphatidylserine as early apoptotic markers: a comparison of two different sperm subpopulations. Fertil Steril 85:149–154CrossRefPubMedGoogle Scholar
- Piasecka M, Laszczynska M, Gaczarzewicz D (2003) Morphological and functional evaluation of spermatozoa from patients with asthenoteratozoospermia. Folia Morphol (Warsz) 62:479–481Google Scholar
- Poongothai J, Gopenath TS, Manonayaki S (2009) Genetics of human male infertility. Singap Med J 50:336–347Google Scholar
- Srivastav A, Chandra A, Singh M, Jamal F, Rastogi P, Rajendran SM, Bansode FW, Lakshmi V (2010) Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant. Reprod Toxicol 29(2):214–224CrossRefPubMedGoogle Scholar
- World Health Organization (1999) WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university pressGoogle Scholar
- World Health Organization. Infertility: a tabulation of available data on prevalence of primary and secondary infertility. Geneva, Switzerland: WHO Programme on Maternal and Child Health and Family Planning; 1991:1–72Google Scholar