Mitochondrial membrane potential (MMP) regulates sperm motility

  • Saurabh Kumar Agnihotri
  • Ankit Kumar Agrawal
  • Bilal Ahmad Hakim
  • A. L. Vishwakarma
  • T. Narender
  • Rekha Sachan
  • Monika Sachdev
Article

Abstract

Sperm motility is the major decisive factor in determining male fertility. The objective of the present study was to analyse the effect of mitochondrial membrane potential (MMP) on the temporal regulation of sperm motility. Observations were recorded in various rodent species and among differentially motile sperm fractions including swim up and leftover layer of human semen sample using JC-1 stain (a marker of the MMP) through FACS. Swim-up sperms having highest motility showed significantly higher MMP as compared to leftover sperms, which had the least motility. Interestingly, infertile patients with compromised motility showed low MMP as compared to the healthy individuals. Further, as per the time lapse, sperm motility goes down, at the same time, it was observed that MMP also decreases in human as well as in rodent sperms. Treatment of known spermicides on human sperms reduced their motility drastically which in turn also reduced its MMP significantly. Treatment of human sperms with oxidative uncoupler also impeded their motility by reducing MMP, indicating a definitive role on MMP on sperm motility and fertility. Based on the results of the study, MMP can be considered as a potential regulator and indicator of sperm motility and hence could be directly related to male fertility.

Keywords

Mitochondrial membrane potential Spermatozoa Sperm motility Infertility 

Notes

Acknowledgments

This research work was financially supported by PROGRAM, Council of Scientific and Industrial Research (CSIR), New Delhi, Government of India. We thank CSIR—Central Drug Research Institute, Lucknow as well as the central FACS facility of the institute.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© The Society for In Vitro Biology 2016

Authors and Affiliations

  • Saurabh Kumar Agnihotri
    • 1
  • Ankit Kumar Agrawal
    • 1
  • Bilal Ahmad Hakim
    • 1
  • A. L. Vishwakarma
    • 2
  • T. Narender
    • 3
  • Rekha Sachan
    • 4
  • Monika Sachdev
    • 1
  1. 1.Endocrinology DivisionCSIR- Central Drug Research InstituteLucknowIndia
  2. 2.Sophisticated Analytical Instrument FacilityCSIR- Central Drug Research InstituteLucknowIndia
  3. 3.Medicinal and Process Chemistry DivisionCSIR- Central Drug Research InstituteLucknowIndia
  4. 4.Department of Obstetrics and GynaecologyKing Georges’ Medical UniversityLucknowIndia

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