Vitamin C stimulates human gingival stem cell proliferation and expression of pluripotent markers

  • Phuc Van Pham
  • Nga Yen Tran
  • Nhan Lu-Chinh Phan
  • Ngoc Bich Vu
  • Ngoc Kim Phan


Gingival stem cells (GSCs) are a novel source of mesenchymal stem cells (MSCs) that are easily accessed from the oral cavity. GSCs were considered valuable autograft MSCs with particular characteristics. However, the limitation in the number of available GSCs remains an obstacle. Therefore, this study aimed to stimulate GSC proliferation by ascorbic acid (AA) and determined the effects of AA on GSC pluripotent potential-related gene expression. GSCs were isolated from gum tissue by explant culture and continuously subcultured before analysis of stemness and effects of AA on pluripotent-related gene expression. GSCs cultured with various concentrations of AA showed increased proliferation in a dose-dependent manner. AA-treated GSCs showed significantly higher expression of SSEA-3, Sox-2, Oct-3/4, Nanog, and TRA-1-60 compared with control cells. More importantly, GSCs also maintained their stemness with MSC phenotypes and failed to cause tumors in nude athymic mice. Our results show that AA is a suitable factor to stimulate GSC proliferation.


Gingival stem cell Gum stem cell Mesenchymal stem cell Pluripotent marker 



This study was funded by Viet Nam National University, Ho Chi Minh City, Viet Nam.

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© The Society for In Vitro Biology 2015

Authors and Affiliations

  • Phuc Van Pham
    • 1
  • Nga Yen Tran
    • 2
  • Nhan Lu-Chinh Phan
    • 1
  • Ngoc Bich Vu
    • 1
  • Ngoc Kim Phan
    • 1
  1. 1.Laboratory of Stem Cell Research and ApplicationUniversity of Science, Viet Nam National UniversityHo Chi Minh CityViet Nam
  2. 2.Faculty of DentistryUniversity of Medicine and PharmacyHo Chi Minh CityViet Nam

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