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In Vitro Cellular & Developmental Biology - Animal

, Volume 51, Issue 8, pp 763–768 | Cite as

Development and characterization of an embryonic cell line from endangered endemic cyprinid Honmoroko Gnathopogon caerulescens (Sauvage, 1883)

  • Shogo Higaki
  • Manami Shimada
  • Yoshie Koyama
  • Yasuhiro Fujioka
  • Noriyoshi Sakai
  • Tatsuyuki Takada
Report

Abstract

Establishing a cell line from endemic species facilitates the cell biological research of these species in the laboratory. In this study, an epithelium-like cell line RME1 was established from the blastula-stage embryos of the critically endangered cyprinid Honmoroko Gnathopogon caerulescens, which is endemic to ancient Lake Biwa in Japan. To the best of our knowledge, this is the first embryonic cell line from an endangered fish species. This cell line is well adapted to grow at 28°C in the culture medium, which was successfully used for establishing testicular and ovarian cell lines of G. caerulescens, and has displayed stable growth over 60 passages since its initiation in June 2011. Although RME1 did not express the genes detected in blastula-stage embryos, such as oct4, sox2, nanog, and klf4, it showed a high euploidy rate (2n = 50; 67.2%) with normal diploid karyotype morphology, suggesting that RME1 retains the genomic organization of G. caerulescens and can prove to be a useful tool to investigate the unique properties of endangered endemic fishes at cellular level.

Keywords

Cell line Embryo Endangered species Fish 

Notes

Acknowledgments

The authors thank personnel of the Shiga Prefectural Fisheries Experimental Station, for providing G. caerulescens embryos. This work was funded in part by a Grant-in-Aid for challenging Exploratory Research (23651248 to T.T.) and Scientific Research on Priority Area (21028021 to T.T.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. This study was also supported by NIG Collaborative Research Program (2010-A41, 2011-A36, and 2012-A28 2013-A47 to T.T.), Ritsumeikan Global Innovation Research Organization (R-GIRO) program (to T.T.) and Center of Innovation Trial Program from Japan Science and Technology Agency, JST (to T.T.).

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Copyright information

© The Society for In Vitro Biology 2015

Authors and Affiliations

  • Shogo Higaki
    • 1
  • Manami Shimada
    • 2
  • Yoshie Koyama
    • 1
  • Yasuhiro Fujioka
    • 3
  • Noriyoshi Sakai
    • 4
  • Tatsuyuki Takada
    • 1
    • 2
    • 5
  1. 1.Ritsumeikan Global Innovation Research OrganizationRitsumeikan UniversityKusatsuJapan
  2. 2.Laboratory of Cell Engineering, Graduate School of Life SciencesRitsumeikan UniversityKusatsuJapan
  3. 3.Lake Biwa MuseumKusatsuJapan
  4. 4.Genetic Strains Research CenterNational Institute of GeneticsMishimaJapan
  5. 5.Laboratory of Cell Engineering, Department of Pharmaceutical SciencesRitsumeikan UniversityKusatsuJapan

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