Development and characterization of an embryonic cell line from endangered endemic cyprinid Honmoroko Gnathopogon caerulescens (Sauvage, 1883)
Establishing a cell line from endemic species facilitates the cell biological research of these species in the laboratory. In this study, an epithelium-like cell line RME1 was established from the blastula-stage embryos of the critically endangered cyprinid Honmoroko Gnathopogon caerulescens, which is endemic to ancient Lake Biwa in Japan. To the best of our knowledge, this is the first embryonic cell line from an endangered fish species. This cell line is well adapted to grow at 28°C in the culture medium, which was successfully used for establishing testicular and ovarian cell lines of G. caerulescens, and has displayed stable growth over 60 passages since its initiation in June 2011. Although RME1 did not express the genes detected in blastula-stage embryos, such as oct4, sox2, nanog, and klf4, it showed a high euploidy rate (2n = 50; 67.2%) with normal diploid karyotype morphology, suggesting that RME1 retains the genomic organization of G. caerulescens and can prove to be a useful tool to investigate the unique properties of endangered endemic fishes at cellular level.
KeywordsCell line Embryo Endangered species Fish
The authors thank personnel of the Shiga Prefectural Fisheries Experimental Station, for providing G. caerulescens embryos. This work was funded in part by a Grant-in-Aid for challenging Exploratory Research (23651248 to T.T.) and Scientific Research on Priority Area (21028021 to T.T.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. This study was also supported by NIG Collaborative Research Program (2010-A41, 2011-A36, and 2012-A28 2013-A47 to T.T.), Ritsumeikan Global Innovation Research Organization (R-GIRO) program (to T.T.) and Center of Innovation Trial Program from Japan Science and Technology Agency, JST (to T.T.).
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