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Use of an in vitro model in tissue engineering to study wound repair and differentiation of blastema tissue from rabbit pinna

  • Mohammad Reza Hashemzadeh
  • Nasser Mahdavi-ShahriEmail author
  • Ahmad Reza Bahrami
  • Masoumeh Kheirabadi
  • Fatemeh Naseri
  • Mitra Atighi
Article

Abstract

Rabbit ear wound repair is an accepted model for studies of tissue regeneration, leading to scar less wound repair. It is believed that a specific tissue, blastema, is responsible for such interesting capacity of tissue regeneration. To test this idea further and to elucidate the cellular events happening during the ear wound repair, we designed some controlled experiments in vitro. Small pieces of the ear were punched and washed immediately with normal saline. The tissues were then cultured in the Dulbecco’s Modified Eagles Medium, supplemented with fetal bovine serum in control group. As a treatment vitamin A and C was used to evaluate the differentiation potency of the tissue. These tissues were fixed, sectioned, stained, and microscopically studied. Micrographs of electron microscopy provided evidences revealing dedifferentiation of certain cells inside the punched tissues after incubation in tissue culture medium. The histological studies revealed that cells of the tissue (i) can undergo cellular proliferation, (ii) differentiate to epithelial, condrogenic, and osteogenic tissues, and (iii) regenerate the wounds. These results could be used for interpretation of the possible events happening during tissue engineering and wound repair in vitro. An important goal of this study is to create a tissue engineering and tissue banking model, so that in the future it could be used in further blastema tissue studies at different levels.

Keywords

Blastema tissue Rabbit pinna Cell culture Differentiation Wound repair 

Notes

Acknowledgments

The authors are thankful to the Institute of Biotechnology, Ferdowsi University of Mashhad for providing work space and facilities. This project was supported by a grant from the Ferdowsi University of Mashhad.

Supplementary material

11626_2015_9868_MOESM1_ESM.docx (15 kb)
ESM 1 (DOCX 14 kb)

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Copyright information

© The Society for In Vitro Biology 2015

Authors and Affiliations

  • Mohammad Reza Hashemzadeh
    • 1
  • Nasser Mahdavi-Shahri
    • 1
    • 2
    Email author
  • Ahmad Reza Bahrami
    • 1
    • 2
  • Masoumeh Kheirabadi
    • 2
  • Fatemeh Naseri
    • 3
  • Mitra Atighi
    • 1
  1. 1.Cell and Molecular Biology Reaserch Group, Institute of BiotechnologyFerdowsi University of MashhadMashhadIran
  2. 2.Department of Biology, Faculty of SciencesFerdowsi University of MashhadMashhadIran
  3. 3.Central LaboratoryFerdowsi University of MashhadMashhadIran

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