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Advantages of assessing lipoprotein profiles in hepatic cell differentiation

  • Junichiro Takahashi
  • Fumiko Kimura
  • Mizuho Miura
  • Yuka Iwama
  • Gen Toshima
  • Keishi Hata
Letter to the Editor

Dear Editor,

Recently, mature hepatocytes have attracted a great deal of attention in drug metabolism or toxicities instead of liver tissue. The human hepatoma cell line, HepG2, was found to chemically differentiate into mature hepatocyte-like cells by sodium (Na) butyrate, and some of the properties of mature hepatocytes were induced by their differentiation (Wang et al. 1998; Fukuda et al. 2005); however, changes in lipid metabolism and lipoprotein production in the cells, which are important functions of hepatocytes, remain unclear.

Lipoproteins are complexes of cholesterol, triglycerides (TG), phospholipids, and apolipoproteins (Apo) and are responsible for transporting lipids in the bloodstream. Serum lipoproteins have been classified into four major classes, chylomicrons, very low density lipoproteins (VLDL), low density lipoproteins, and high density lipoproteins (HDL) by their density and size. The liver is a major organ for lipid metabolism and mainly secretes serum lipids as...

Keywords

HepG2 Cell Butyrate Squalene High Density Lipoprotein Human Hepatoma Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Conflict of interest

We declare that we have no conflict of interest in the present study.

Supplementary material

11626_2013_9639_MOESM1_ESM.doc (428 kb)
ESM 1 (DOC 427 kb)

References

  1. Fukuda J.; Okamura K.; Ishihara K.; Mizumoto M.; Nakazawa K.; Ijima H.; Kajiwara T.; Funatsu K. Differentiation effects by the combination of spheroid formation and sodium butyrate treatment in human hepatoblastoma cell line (HepG2): a possible cell source for hybrid artificial liver. Cell Transplant 14: 819–827; 2005.PubMedCrossRefGoogle Scholar
  2. Itoh M.; Abe Y.; Iwama Y.; Kimura F.; Satoh M.; Shoji M.; Takahashi J.; Toshima G.; Sasaki H.; Hiwatashi K.; Hata K. HPLC analysis of lipoproteins in culture medium of hepatoma cells: an in vitro system for screening antihyperlipidemic drugs. Biotechnol Lett 31: 953–957; 2009.PubMedCrossRefGoogle Scholar
  3. Takahashi J.; Toshima G.; Matsumoto Y.; Kimura F.; Kiuchi T.; Hamada K.; Hata K. In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells. J Nat Med 65: 670–674; 2011.PubMedCrossRefGoogle Scholar
  4. Wang X. M.; Wang X.; Ling B. S. J.; Evers B. M. Effect of 5-azacytidine and butyrate on differentiation and apoptosis of hepatic cancer cell lines. Ann Surg 214: 922–931; 1998.CrossRefGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2013

Authors and Affiliations

  • Junichiro Takahashi
    • 1
  • Fumiko Kimura
    • 1
  • Mizuho Miura
    • 1
  • Yuka Iwama
    • 1
  • Gen Toshima
    • 1
  • Keishi Hata
    • 2
  1. 1.Skylight Biotech IncAkitaJapan
  2. 2.Akita Research Institute of Food and BrewingAkitaJapan

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