Advantages of assessing lipoprotein profiles in hepatic cell differentiation
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Recently, mature hepatocytes have attracted a great deal of attention in drug metabolism or toxicities instead of liver tissue. The human hepatoma cell line, HepG2, was found to chemically differentiate into mature hepatocyte-like cells by sodium (Na) butyrate, and some of the properties of mature hepatocytes were induced by their differentiation (Wang et al. 1998; Fukuda et al. 2005); however, changes in lipid metabolism and lipoprotein production in the cells, which are important functions of hepatocytes, remain unclear.
Lipoproteins are complexes of cholesterol, triglycerides (TG), phospholipids, and apolipoproteins (Apo) and are responsible for transporting lipids in the bloodstream. Serum lipoproteins have been classified into four major classes, chylomicrons, very low density lipoproteins (VLDL), low density lipoproteins, and high density lipoproteins (HDL) by their density and size. The liver is a major organ for lipid metabolism and mainly secretes serum lipids as...
KeywordsHepG2 Cell Butyrate Squalene High Density Lipoprotein Human Hepatoma Cell
Conflict of interest
We declare that we have no conflict of interest in the present study.
- Fukuda J.; Okamura K.; Ishihara K.; Mizumoto M.; Nakazawa K.; Ijima H.; Kajiwara T.; Funatsu K. Differentiation effects by the combination of spheroid formation and sodium butyrate treatment in human hepatoblastoma cell line (HepG2): a possible cell source for hybrid artificial liver. Cell Transplant 14: 819–827; 2005.PubMedCrossRefGoogle Scholar
- Itoh M.; Abe Y.; Iwama Y.; Kimura F.; Satoh M.; Shoji M.; Takahashi J.; Toshima G.; Sasaki H.; Hiwatashi K.; Hata K. HPLC analysis of lipoproteins in culture medium of hepatoma cells: an in vitro system for screening antihyperlipidemic drugs. Biotechnol Lett 31: 953–957; 2009.PubMedCrossRefGoogle Scholar