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Embryo–luteal cells co-culture: an in vitro model to evaluate steroidogenic and prostanoid bovine early embryo–maternal interactions

  • Ana Torres
  • Mariana Batista
  • Patrícia Diniz
  • Luisa Mateus
  • Luís Lopes-da-Costa
Article

Abstract

The role of progesterone (P4) and prostaglandins (PGs) in bovine early embryonic development and embryo–maternal crosstalk is almost unknown. Here, the in vitro steroidogenic (P4) and prostanoid (PGE2 and PGF2α) interactions between bovine embryos and luteal cells (LC) were evaluated. In two experiments, embryos (n = 1.900) were either co-cultured with LC or cultured alone, from days 2 to 7 (day 0 = in vitro insemination). LC were also cultured alone, and medium was used as a control, all groups being cultured either with or without oil overlay of culture medium. Oil overlay of culture medium significantly decreased the amount of P4, but not of PGE2 and PGF measured in culture medium. Embryos and LC had transcripts of genes coding for enzymes of the PGs (PTGS2, PGES, and PGFS) and P4 (StAR, P450scc, and 3β-HSD) synthesis pathways, and produced P4, PGF, and PGE2 into culture medium. Co-culture with LC exerted an embryotrophic effect, significantly increasing blastocyst yield and quality. This indicates a possible direct effect of LC in early embryo development. Embryos did not exert a luteotrophic effect upon LC. This may indicate that early embryos (until day 7) probably do not exert influence in LC main function. It is suggested that production of P4, PGE2, and PGF by early embryos may be associated to autocrine signaling leading to events in development and to paracrine signaling in the endometrium leading to local uterine receptivity.

Keywords

Embryo Luteal cells Co-culture Progesterone Prostaglandins Bovine 

Notes

Acknowledgments

This study was funded by the grant PTDC/CVT/65690/2006 from Foundation for Science and Technology (FCT). Ana Torres was a Ph.D. fellow from FCT (SFRH/BD/37666/2007), and Mariana Batista had a research fellowship funded by the above grant. The authors thank Dr. Elisabete Silva for her assistance in real-time PCR analysis.

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Copyright information

© The Society for In Vitro Biology 2012

Authors and Affiliations

  • Ana Torres
    • 1
  • Mariana Batista
    • 1
  • Patrícia Diniz
    • 1
  • Luisa Mateus
    • 1
  • Luís Lopes-da-Costa
    • 1
  1. 1.Reproduction and Obstetrics, CIISA, Faculty of Veterinary MedicineTechnical University of LisbonLisbonPortugal

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