Effects of anti-lipid peroxidases on frozen-thawed boar spermatozoa

  • Sarah J. Casey
  • Rachel Taupier
  • Brian D. Whitaker


This study evaluated the effects of anti-lipid peroxidases when supplemented to the thawing and incubation media of frozen-thawed boar spermatozoa. Semen pellets were thawed and incubated in media with 1.0 mM α-tocopherol or diethylenetriamine. After 1 h, the acrosome reaction was induced using calcium ionophore A23187, and acrosomes were evaluated using Wells–Awa staining. The number of spermatozoa with fragmented DNA was evaluated using silver staining after single-cell gel electrophoresis. Membrane lipid peroxidation was measured by the end point generation of malondialdehyde. The diethylenetriamine-supplemented media had a higher (P < 0.05) percentage of acrosome-reacted spermatozoa (84.4 ± 4.1%) compared to the control (78.3 ± 4.2%) and α-tocopherol-supplemented (78.0 ± 3.9%). The control had a higher (P < 0.05) percentage of spermatozoa with fragmented DNA (59.3 ± 4.3%) compared to the DETA (28.7 ± 4.1%) and α-tocopherol supplementation (28.0 ± 3.8%). Spermatozoa supplemented with diethylenetriamine had higher amounts (P < 0.05) of malondialdehyde generated (3.60 ± 0.05 μM/107 cells) compared to the α-tocopherol supplementation (0.14 ± 0.05 μM/107 cells) and the control (0.12 ± 0.05 μM/107 cells). These results indicate that supplementing with either 1.0 mM diethylenetriamine or α-tocopherol during semen thawing and incubation protects against DNA fragmentation, and diethylenetriamine increases the percent of spermatozoa capable of completing the acrosome reaction that could induce membrane lipid peroxidation.


Sperm Anti-oxidants α-Tocopherol Lipid peroxidation 


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Copyright information

© The Society for In Vitro Biology 2011

Authors and Affiliations

  • Sarah J. Casey
    • 1
  • Rachel Taupier
    • 1
  • Brian D. Whitaker
    • 2
  1. 1.Department of BiologyFerrum CollegeFerrumUSA
  2. 2.Department of Animal SciencesUniversity of FindlayFindlayUSA

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